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Calmodulin-SH, Wheat (Triticum aestivum); CaMTag™ DuoSite
[C-1421]
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Product Name: Calmodulin-SH, Wheat (Triticum aestivum);
CaMTag™ DuoSite
Product #: C-1421
Size & Price: 250 ug @ $60.00, 1 mg @ $160.00
Description:
A sequence ideal for applications requiring both calmodulin activation and residue specific tagging.
Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions and calcium-calmodulin signal transduction pathways. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response.
Calmodulin-SH, Wheat (Triticum aestivum).
Wheat germ (Triticum aestivum) calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26). In contrast to mammalian CaMs, which lack cysteine, this preparation is ideal for applications requiring both calmodulin activation and residue specific tagging. The single SH moiety at Cys26 can be labeled using a variety of sulfhydryl (SH) reactive protein-modification reagents such as fluorescent maleimide derivatives. Additionally, since wheat germ CaM contains only a single tyrosine residue (Tyr138) verses two tyrosine residues found in mammalian CaM, Tyr138 serves as a mono-specific C-terminal region labeling site.
Using Cys26 and Tyr138 allows wheat germ CaM to be independently derivatized at both N- and C-terminal regions within the molecule for structural, proteomic biomarker discovery and protein-protein interaction studies. Wheat germ CaM also has no tryptophan resulting in a distinctive absorbance spectrum when compared to mammalian CaM sequences.
Swiss-Prot Entry: P04464
Active Product: Yes
Appearance: Lyophilized from 5% sucrose, 10 mM sodium phosphate, pH 7.0 at a protein concentration of approximately 1 mg per ml.
Purity: This preparation is greater than 98% by SDS-PAGE stained with Coomassie blue having a single band at approximately 17 kDa; Actual molecular weight is 16,750 Da.
Activity: Greater than 35,000 units per mg protein. One unit will stimulate 0.01 unit of 3':5'-cyclic nucleotide phosphodiesterase in a 3 ml reaction volume at pH 7.5 and 30C, to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 uM Ca2+.
Handling: Store in Tightly Sealed Vial.
Storage: -20C.
Shipping: Express Courier
General Comments: Products are for scientific research use only.
Reference: Strasburg, G.M., et al. Site-specific derivatives of wheat germ calmodulin. Interactions with troponin and sarcoplasmic reticulum. J Biol Chem. 1988. 263:12161.
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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