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Calmodulin-SH, Wheat (Triticum aestivum), Rhodamine; CaMTag™ Rhodamine
[C-1427]
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Product Name: Calmodulin-SH, Wheat (Triticum aestivum), Rhodamine; CaMTag™ Rhodamine
Product # C-1427
Size & Price: 50 ug @ $250.00
Description: Calmodulin, Wheat (Triticum aestivum), Rhodamine Wheat germ (Triticum aestivum) is unique in that it contains cysteine at residue position 26 (Cys26). In contrast to mammalian CaMs, which lack cysteine, this preparation is ideal for applications requiring both calmodulin activation and residue specific tagging. Mono-tagged rhodamine labeled wheat germ calmodulin at position Cysteine 26 is useful for protein array applications for the detection of calmodulin binding proteins (CBPs), Western blots and other applications requiring a convenient and superior fluorescently labeled calmodulin.
Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions and calcium-calmodulin signal transduction pathways. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response.
Swiss-Prot Entry: P04464
Active Product: Yes
Appearance: Lyophilized from 5% sucrose, 10 mM sodium phosphate, pH 7.0 at a protein concentration of approximately 1 mg per ml in a dark plastic screw-cap 1.5 ml vial.
Purity: This preparation is greater than 98% by SDS-PAGE stained with Coomassie blue having a single band at approximately 17 kDa; Actual molecular weight is 16,750 Da.
Activity: Greater than 35,000 units per mg protein. One unit will stimulate 0.01 unit of 3':5'-cyclic nucleotide phosphodiesterase in a 3 ml reaction volume at pH 7.5 and 30C, to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 uM Ca2+.
Fluorochrome/Protein Ratio: Rhodamine/CaM = 0.5 to 1.0 as determined by absorbance.
Handling: Store in Tightly Sealed Vial. Protect from Light.
Storage: -20C.
Shipping: Express Courier
General Comments: Products are for scientific research use only.
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$250.00
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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