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Calmodulin-SH, Wheat (Triticum aestivum), Immobilized High Loading; CaMTag™ HD Capture
[C-1429]
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Product Name: Calmodulin-SH, Wheat (Triticum aestivum), Immobilized High Loading; CaMTag™ HD Capture
Product # C-1429
Size & Price: 200 ul @ $250.00
Description: Calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26). Wheat germ calmodulin coupled primarily through SH group (Cys26) to epoxy activated resin. Coupled at 5 mg per mL and useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles. Loading conditions: 100 uM Ca2+, with protease inhibitors and appropriate buffers. Elution conditions: Buffers with 1 mM EDTA or EGTA.
Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions and calcium-calmodulin signal transduction pathways. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response.
Active Product: Yes
Appearance: 50% slurry in 3 M NaCl, 20mM sodium phosphate buffer, pH, 7.4 and 1 mM EGTA.
Purity: Calmodulin Content: 1 mL of resin bound with 5 –5.5 mg of wheat germ calmodulin.
Handling: Store in Tightly Sealed Vial.
Storage: +4°C. Do not freeze.
Shipping: Express Courier
General Comments: Products are for scientific research use only.
Reference: Strasburg, G.M., et al. Site-specific derivatives of wheat germ calmodulin. Interactions with troponin and sarcoplasmic reticulum. J Biol Chem. 1988. 263:12161.
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$250.00
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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