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Calmodulin, Porcine, Immobilized Agarose
[C-1439]
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Product Name: Calmodulin, Porcine, Immobilized Agarose
Product # C-1439
Size & Price: 2 mL packed resin @ $300.00
Description: Calmodulin, Porcine, Immobilized Agarose Mammalian calmodulin immobilized to aldehyde activated agarose at 1 mg per mL. Coupled at 5 mg per mL and useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles. Loading conditions: 100 uM Ca2+, with protease inhibitors and appropriate buffers. Elution conditions: Buffers with 1 mM EDTA or EGTA.
Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions and calcium-calmodulin signal transduction pathways. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response.
Appearance: 50% slurry in 3 M NaCl, 20mM sodium phosphate buffer, pH, 7.4 and 1 mM EGTA.
Purity: Calmodulin Content: 1 mL of resin bound with 5 –5.5 mg of porcine calmodulin.
Handling: Store in Tightly Sealed Vial.
Storage: +4C.
Shipping: Express Courier
General Comments: The animal source of this product was collected at a USDA licensed establishment. The animals received ante and post mortem health inspection at the abattoir by a US FSIS inspector and they were apparently free from infectious and contagious diseases. All donor animals were sourced from the United States.
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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