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Calmodulin-SH, Wheat (Triticum aestivum) & Porcine, Immobilized High Loading; CaMTag™ HD Capture plus
[C-1441]
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Product Name: Calmodulin-SH, Wheat (Triticum aestivum) & Porcine, Immobilized High Loading; CaMTag™ HD Capture plus
Product # C-1441
Size & Price: 200 uL packed resin @ $245.00
Description:
Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions and calcium-calmodulin signal transduction pathways. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response
Calmodulins immobilized via SH (wheat germ) and amino groups (porcine) to epoxy resin allowing multiple orientations of bound calmodulin for efficient capture of calmodulin binding proteins. Wheat germ (Triticum aestivum) calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26).
Wheat germ calmodulin coupled primarily through SH group (Cys26) to epoxy activated resin. Coupled at 5 mg per mL and useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles. Loading conditions: 100 uM Ca2+, with protease inhibitors and appropriate buffers. Elution conditions: Buffers with 1 mM EDTA or EGTA
Appearance: 50% slurry in 3 M NaCl, 20mM sodium phosphate buffer, pH, 7.4 and 1 mM EGTA.
Purity: Calmodulin Content: 200 uL of resin bound with 1 – 1.1 mg of calmodulin.
Handling: Store in Tightly Sealed Vial.
Storage: +4°C. Do not freeze.
Shipping: Express Courier
General Comments: The animal source of this product was collected at a USDA licensed establishment. The animals received ante and post mortem health inspection at the abattoir by a US FSIS inspector and they were apparently free from infectious and contagious diseases. All donor animals were sourced from the United States.
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$245.00
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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