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Monoclonal Antibody to Beta-Catenin
[C1202]
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PRODUCT: Monoclonal Antibody b-Catenin (dephospho) (8E4)
(includes Positive Control )
LOT NUMBER: A6290
PRODUCT NUMBER: C1202
CLONE: 8E4 SIZE: 100 UG
HOST: Mouse
ISOTYPE: lgG1 kappa
IMMUNOGEN:Synthetic Peptide
EPITOPE: aa 27-37
SPECIES REACTIVITY:Human, Mouse, and Rat, others not determined
BACKGROUND & SPECIFICITY:
The a-, b-, and g-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of b-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighboring cells. Recent studies indicate that b-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. b-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that b-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity. Mab b-CAT-8E4 specifically interacts with nuclear b-catenin.
PURIFICATION:The antibody was purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:Lyophilized from 1 ml 2 x PBS/ 0.1% Na-azide/ PEG and Sucrose.
RECONSTITUTION: Reconstitute with 1 ml H2O (15 Min, RT)
STABILITY:Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Avoid repeated freeze / thaw cycles
IMMUNOBLOTTING: 1 mg/ml for AP/BCIP/NBT (MTT) detection; 0.1 mg/ml for HRPO/ECL detection
IMMUNOPRECIPITATION:Use 1-10 ug per 106 vanadate treated A431 cells
ELISA: Use at 0.05 mg/ml
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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