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Hygromycin B, Powder
[H-1012-SOLID]
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Description: Hygromycin B, an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus, is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells. Sterile filtered in DI Water.
Chemical Formula: C20H37N3O13
Chemical Name: : O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-beta-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
Molecular Weight: M.W. 527.53
Solubility: Hygromycin is soluble in Water.
CAS Number: CAS [31282-04-9]
Active Product: Yes
Appearance: White to Off White Solid
Purity: >90%
Handling: Warning! Toxic / Harmful. May be Carcinogenic / Teratogenic. Working hygromycin solutions (<2mg/ml) are stable for ~1 month at +4°C.
Storage: +4°C.
Shipping: Express Courier
General Comments: The working Hygromycin B concentration, for the purpose of selection, varies with cell type, media, growth conditions and cell metabolic rate. Recommended hygromycin concentration for the selection of resistant cells is 25-1000 ug/ml. Commonly used concentrations for selection are 200 ug/ml for mammalian cells, 20-200 ug/ml for plant cells & bacteria cells and 200-1000 ug/ml for fungi. Your optimum hygromycin concentration should be tested experimentally.
Bulk Quantity: Readily available, inquire for pricing.
A.G. Scientific also offers Hygromycin B in DI water, PBS, or concentrated solution (1MU).
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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