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HRV3C Protease, Human rhinovirus 3C protease
[H-1192]
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Description: HRV3C Protease, (Human rhinovirus 3C protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly. The recombinant form of the HRV3C protease is a restriction grade protease that has robust activity at 4°C and high specific activity. This HRV 3C Protease is a 47 kDa protein with both GST and His tags so it can be removed by either Ni-chelating or Glutathione (GSH) resin
Activity and Specificity: The activity of HRV 3C Protease is tested using a control cleavage protein. One ug of HRV 3C Protease has an equivalent of 1 unit activity conventionally used by other suppliers (1 unit of HRV3C protease cleaves >95% target cleavage protein at 4°C for 16 hours.).
Component: One mg of HRV 3C Protease in 500 ul at 2 mg/ml in a storage buffer of 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM TCEP, and 50% glycerol.
Clevage condition: It is recommended to test the HRV3C Protease cleavage with protease-to-target protein ratio of 1:100 (w/w) in the buffer suitable for the target protein at 4°C overnight, with the target protein concentration at or below 1 mg/ml. Under this condition, > 95% of target protein is cleaved in most cases. The efficiency of cleavage varies due to the sequences around the cleavage site, the conformation and the solubility of the target protein. Including reducing agent such as DTT or TCEP in the buffer helps the cleavage. Due to its high specificity, more protease can be used without non-specific cleavage of target proteins. No non-specific cleavage of control cleavage protein is observed at protease-to-control cleavage protein ratio of 1:10.
Removal of HRV 3C Protease after Cleavage The HRV 3C Protease contains both GST and His tags. After cleavage of the target protein, HRV3C Protease can be easily removed along with the tags from the cleavage reaction by affinity chromatography on a Ni-chelating resin for His-tagged target protein or GSH resin for GST-tagged target proteinExpress Courier
Bulk Quantity: Available
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$200.00
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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