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ISG15 Activating Enzyme (E1), Human
[I-1300]
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This E1 enzyme is responsible for teh first step in ISG15-protein ispeptide bond formatioin. The exzyme catalyzes the activation of the -terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The ISG15 enzyme is a critical component for the initiation of any in vitro conjugation reactions.
Formulation: Solution in 20% glycerol, 150mM NaCl, 5mM DTT, 75mM Tri-HCl, pH 8.0
Concentration: 0.5 ug/ul
Purity: >= 95% (SDS-PAGE)
MW: 138 kDa
Storage: -80C, avoid freeze-thaw. Shipped on dry ice.
Sizes: 50ug (GST tagged) or 25ug (un-tagged)
Lit Refs: inquire for referene detail.
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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