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Monoclonal Antibody to Nitrosocysteine, Purified
[N1078]
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Used to localize and characterize S-nitrosylated proteins in cells in and tissues. Antibody was obtained after BALB/c mouse immunization with the conjugates: NO-Cysteine-Glutaraldehyde-Carrier proteins and hybridization of spleen cells with the myeloma cell line SP2/O/Ag14. Ascite production was performed in BALB/c mice.
Applications: In vivo administration, Immunohistochemistry Immunoblotting, Western Blot
Storage and Handling:
Purified antibodies lyophilized & freeze dried with 0.01% merthiolate; sufficient for 200 tests.
Store as supplied at -20C. Reconstitute with 25ul or 50ul of distilled water, store reconsituted at 4C.
Specificity
Using a conjugate NO-Cystéine-Glutaraldehyde(G)-Protein antibody specifity was performed with an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio (a)
L.Cysteine-G-BSA, 1/200
NO-NAC-BSA 1/>50,000
NAC-BSA 1/>50,000
(a) : NO-Cystéine-G-BSA concentration/unconjugated or conjugated catechloamine concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin
Recommanded dilution:
The anti-conjugated NO-Cystéine antibodies produced good staining at 1/2000-1/5000 dilution in these areas.
Subclass: IgG1, Kappa
Purification: Ascitic fluid was purified by ammonium sulfate precipitation and gel filtration.
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Note: All products sold by A.G. Scientific are for Laboratory or Manufacturing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
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