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SUMO Activating Ennzyme (E-Uba2 & E-Aos1), Human
[S-2074]
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Conjugation of the ubiquitin-like modifier SUMO-1 requires the activities of the heterodimeric E1 (Aos1/Uba2) enzyme and the Ubc9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of SUMO-1, forming a high-energy thiolester bond with the cysteine residue of Uba2 and releasing AMP. The second step is a trans-esterification reaction whereby SUMO-1 is transferred to the Cys 93 residue of Ubc9. Produced by Proteome.
Formulation: Solution in 20% glycerol, 150mM NaCl, 5mM DTT, 75mM Tris-HCl, pH 8.0
Concentration: 0.5 ug/ul
MW: Aos1 ~40 kDA; Uba2 ~ 80 kDA
Storage: -80C, avoid freeze-thaw cycles. Shipped on dry ice.
Sizes: 25ug (untagged) or 50ug (GST tagged)
Literature References: inquire for reference list.
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