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Blasticidin S . HCl, CAS 3513-03-9, Selection Antibiotic, Blasticidin S, is used to select transfected cells carrying bsr or BSD resistance genes. A.G. Scientific supplies unique or hard to find antibiotics

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Blasticidin S.HCl Solution

CAS: 3513-03-9 Formula: C17H26N8O5.HCl MW: 458.9 Appearance: Liquid, 10 mg / ml in Hepes buffer (100 mg total) Purity: ≥98.0%
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PRODUCT # SIZE LIST PRICE QUANTITY BUY NOW BULK INQUIRIES
B-1247-SOL 5x 2 ml
$260.00
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Blasticidin S is an aminoacylnucleoside antibiotic isolated from Streptomyces griseochromogenes. It shows high efficacy against both eukaryotic and prokaryotic cells. Aside from its antimicrobial properties, blasticidin has displayed antiviral, antitumor, and antifungal properties and has been used as a selection agent. It has been used to select for cells containing the blsbsr, or BSD resistance gene as well as HEK293-T cells and HEK-D5 cells. Additionally, blasticidin is effective in preventing cell culture contamination and studying protein synthesis.

Blasticidin S functions by inhibiting peptide bond formation at the ribosome. It blocks hydrolysis of peptidyl-tRNA due to the large ribosomal subunit's increased affinity for tRNA.

Blasticidin S, longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system1,2. Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in Duchenne muscular dystrophy (DMD)3.

1.)   http://www.genetics.org/content/genetics/197/4/1069.full.pdf

2.)   http://www.g3journal.org/content/early/2016/04/11/g3.116.028241.full.pdf+html

3.)    http://nar.oxfordjournals.org/content/44/3/1449.full.pdf+html

Additional Information

Product # B-1247-SOL
CAS # 3513-03-9
Formula C17H26N8O5.HCl
MW 458.9
Appearance Liquid, 10 mg / ml in Hepes buffer (100 mg total)
Purity ≥98.0%
Solubility Clear and faint yellow solution (5mg/ml in water)
Storage Temp 20°C

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Merck Index 14, 1316

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Additional Information

msds 1 B-1247, Blasticidin S HCl Solution, MSDS.pdf
Certificate of Analysis 1 B-1247-SOL, G1218.pdf
Certificate of Analysis 2 B-1247-SOL, G1217.pdf
Certificate of Analysis 3 B-1247-SOL, G1052.pdf
E-Brochure Antibiotics Brochure

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Blasticidin S.HCl Solution

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