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Mammalian calmodulin immobilized to aldehyde activated agarose at 1 mg per mL. Coupled at 5 mg per mL and useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles. Loading conditions: 100 μM Ca2+, with protease inhibitors and appropriate buffers. Elution conditions: Buffers with 1 mM EDTA or EGTA.
|Appearance||50% slurry in 3 M NaCl, 20mM sodium phosphate buffer, pH, 7.4 and 1 mM EGTA.|
|Purity||Calmodulin Content: 1 mL of resin bound with 5 –5.5 mg of porcine calmodulin.|
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