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Mammalian calmodulin labeled with NHS ester of rhodamine is a ubiquitous, calcium-binding protein that binds and regulates a multitude of protein targets, many of which are involved in the Alzheimer's and Parkinson's pathways.
|Appearance||Lyophilized from 5% sucrose, 10 mM sodium phosphate, pH 7.0 at a protein concentration of approximately 1 mg per ml in a dark plastic screw-cap 1.5 ml vial.|
|Purity||Physical Purity: > 98% by SDS-PAGE stained with Coomassie blue having a single band at approximately 17 kDa. Activity: Greater than 35,000 units per mg protein. One unit will stimulate 0.01 unit of 3′:5′-cyclic nucleotide phosphodiesterase in a 3 ml reaction volume at pH 7.5 and 30°C, to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 μM Ca2+. Fluorochrome/Protein Ratio: Rhodamine/CaM = 0.5 to 1.0 as determined by absorbance.|
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