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Product Description
Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Additional Information
| Product Number | C-2239 |
| CAS # | N/A |
| Chemical Name | Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic |
| Chemical Formula | C30H33F3N5O13 |
| Appearance | Liquid |
| Molecular Weight | 729 |
| Purity | >98% by HPLC |
| Solubility | N/A |
| Storage Temp | -20°C |
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All products sold by A.G. Scientific are strictly for Laboratory, Preclinical Research, or Bioprocessing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
