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Product Description
Ready-to-use fluorometric substrate for caspase-1,-4,-5 and related caspases that recognize the amino acid sequence WEHD. Caspase-5 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate WEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) for caspase assays are also available separately.
Additional Information
| Product Number | C-2245 |
| CAS # | N/A |
| Chemical Name | Ac-WEHD-AFC, Caspase-5 Substrate, Fluorogenic |
| Chemical Formula | C38H37F3N8O11 |
| Appearance | Liquid |
| Molecular Weight | 838.8 |
| Purity | >99% by HPLC |
| Solubility | N/A |
| Storage Temp | -20°C |
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All products sold by A.G. Scientific are strictly for Laboratory, Preclinical Research, or Bioprocessing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
