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Product Description
Cell permeable analog of DAF-2 that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in nitric oxide (NO) levels in vivo (Ex(max): 495nm; Em(max): 515nm).
Prepare the reagent just before use. Use immediately after dilution and do not store this solution for later use.
After opening, the unused (undiluted) portion of the reagent should be aliquoted (10 ml size) in separate tubes and stored in the refrigerator, protected from light.
The dilution buffer should have a pH of 7-7.5. Bovine serum albumin (BSA), phenol red and amines may affect the fluorescence and thus should be used with caution.
Additional Information
COA Documentation: |
|
| Product Number | D-1149 |
| Chemical Name | 4,5-Diaminofluorescein.diacetate. The reagent is supplied at a concentration of ~5mM. Prepare a 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) at time of use. |
| Chemical Formula | C24H18N2O7 |
| Alternative Name | DAF-2 DA, Cell Permeable | Measures real-time changes in Nitric Oxide |
| Appearance | Liquid, 1 mg in 0.45 ml DMSO. |
| Merck Index | Merck Index: 13.2829.2001 |
| Molecular Weight | M.W. 446.4 |
| Preparation | Use product immediately after dilution and do not store the solution for later use. Use at pH 7.0-7.5. |
| Purity | Purity: ≥ 99.5% |
| Storage Temp | +4°C |
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