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Product Description
Wheat germ (Triticum aestivum) calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26). In contrast to mammalian CaMs, which lack cysteine, this preparation is ideal for applications requiring both calmodulin activation and residue specific tagging. Mono-tagged fluorescein labeled wheat germ calmodulin at position Cysteine 26 is useful for protein array applications for the detection of calmodulin binding proteins (CBPs), Western blots and other applications requiring a convenient and superior fluorescently labeled calmodulin.
Additional Information
| Product Number | C-1425 |
| CAS # | Swiss-Prot Entry: P04464 |
| Alternative Name | CaMTag™ Fluorescein |
| Appearance | Lyophilized from 5% sucrose, 10 mM sodium phosphate, pH 7.0 at a protein concentration of approximately 1 mg per ml in a dark plastic screw-cap 1.5 ml vial. |
| Molecular Weight | 16,750 Da |
| Purity | Physical Purity: This preparation is greater than 98% by SDS-PAGE stained with Coomassie blue having a single band at approximately 17 kDa; Actual molecular weight is 16,750 Da.Activity: Greater than 35,000 units per mg protein. One unit will stimulate 0.01 unit of 3′:5′-cyclic nucleotide phosphodiesterase in a 3 ml reaction volume at pH 7.5 and 30°C, to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 μM Ca2+.Fluorochrome/Protein Ratio: fluorescein /CaM = 0.5 to 1.0 as determined by absorbance. |
| Storage Temp | -20°C. |
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All products sold by A.G. Scientific are strictly for Laboratory, Preclinical Research, or Bioprocessing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
