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Produced in HEK 293 cells. The extracellular domain of human Fas (CD95; APO-1) (aa 7-154) is fused to the Fc portion of human IgG1. Inhibits the activity of human and mouse soluble Fas (sFasL) and thereby sFasL-mediated lysis of Fas sensitive cells in a concentration range of 20-100µg/ml. Kit contains Enhancer, which increases the inhibitory activity by ~20- to 50-fold to a concentration range of 0.5-5µg/ml. Specificity: Fas:Fc binds human, mouse and rat FasL (APO-1L; CD95L; CD178). F-1068 = Fas (human):Fc (human) (recombinant) only, without enhancer.
|Alternative Name||APO-1 (human):Fc (human) KIT , TNFRSF 6 (human):Fc (human) (recombinant) KIT|
|Appearance||Lyophilized. Contains PBS.|
|Solubility||1mg/ml after reconstitution. Reconstitute Fas:Fc with 50µl and enhancer with 20µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.|
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