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G-418.Sulfate, CAS 108321-42-2, G418, Aminoglycoside antibiotic, is used as a selective agent of transfected mammalian, yeast, plant and bacteria cells. Resistance is conferred by the bacterial gene for aminoglycoside-3'-phophotransferase that can be ex

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G-418.Sulfate

CAS: 108321-42-2 Formula: C20H40O10N4.2H2SO4 MW: 692.7 Appearance: Off-white to off-white powder Purity: >700 μg/mg
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G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker.

G418 disulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml.  It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through thenptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells.

G418 longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system1.

1. http://femsyr.oxfordjournals.org/content/femsyr/15/2/fov004.full.pdf

CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.

 

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Synonyms Geneticin®
Product # G-1033
CAS # 108321-42-2
Formula C20H40O10N4.2H2SO4
MW 692.7
Appearance Off-white to off-white powder
Purity >700 μg/mg
Solubility Soluble in Water and Aqueous Buffers
Storage Temp +4°C

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GHS Pictograms
RTECS CB9378500

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msds 1 G-1033 SDS.pdf
Certificate of Analysis 1 G-1033, G1274.pdf
Certificate of Analysis 2 G-1033, G1171.pdf
Certificate of Analysis 3 G-1033, F1294C.pdf

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±7 Interfaces, Teresa A. Murray, Daniel Bertrand, et al, Mol. Pharmacol., Feb 2012. 81: 175 - 188.
Role of MRE11 in Cell Proliferation, Tumor Invasion, and DNA Repair in Breast Cancer, Shyng-Shiou F. Yuan, Ming-Feng Hou, et al, J Natl Cancer Inst, Oct 2012; 104: 1485 - 1502.
Targeting of the Orphan Receptor GPR35 by Pamoic Acid: A Potent Activator of Extracellular Signal-Regulated Kinase and Arrestin 2 with Antinociceptive Activity, Pingwei Zhao et al, Molecular Pharmacology October 2010. 78: 560-568.
PKC and cofilin activation affects peripheral actin reorganization and cell-cell contact in cells expressing integrin 5 but not its tailless mutant. Min-A Oh et al, J Cell Sci., August 1, 2007. 120: 2717-2730
Molecular Mechanism of Constitutive Endocytosis of Acid-Sensing Ion Channel 1a and Its Protective Function in Acidosis-Induced Neuronal Death., Wei-Zheng Zeng et al, The Journal of Neuroscience, 17 April 2013, 33(16):7066-7078.
Endolysosomal Membrane Trafficking Complexes Drive Nutrient-Dependent TORC1 signaling to Control Cell Growth in Saccharomyces cerevisiae. Kingsbury, J. M. et al. Genetics. Apr 2014. 196: 1077-1089.
FUNGAL AND PARASITIC INFECTIONS: Discovery of a Role for Hsp82 in Histoplasma Virulence through a Quantitative Screen for Macrophage Lethality. Edwards, J. A. et al. Infect. Immun. Aug 2011. 79:3348-3357.
VIRUS-CELL INTERACTIONS: Epstein-Barr Virus SM Protein Utilises Cellular Splicing Factor SRp20 To Mediate Alternative Splicing. Verma, D. et al. J. Virol. Nov 2010. 84: 11781-11789.
TRP14 Inhibits Osteoclast Differentiation Via Its Catalytic Activity. Hong, S. et al. Mol. Cell. Biol. Sep 2014. 34: 3515-3524.
PATHOGENESIS AND IMMUNITY: Hepatitis C Virus Entry is Impaired by Claudin-1 Downregulation in Diacylglycerol Acyltransferase-1-Deficient Cells. Sung, P. S. et al. J. Virol. Aug 2014. 88: 9233-9244.
CANCER THERAPY PRECLINICAL: Contribution of OATP1B3 to the Deposition of Sorafenib and Sorafenib-Glucuronide. Zimmerman, E. I. et al. Clin. Cancer Res. March 2013. 19: 1458-1466.
Sphingosine 1-phosphate and human ether-a'-go-go-related gene potassium channels modulate migration in human anaplastic thyroid cancer cells. Asghar, M. Y. et al. Endrocr. Relat. Cancer. Sep 2012. 19:667-680.

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G-418.Sulfate

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