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G-418.Sulfate, CAS 108321-42-2, G418, Aminoglycoside antibiotic, is used as a selective agent of transfected mammalian, yeast, plant and bacteria cells. Resistance is conferred by the bacterial gene for aminoglycoside-3'-phophotransferase that can be ex

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G-418.Sulfate

CAS: 108321-42-2 Formula: C20H40O10N4.2H2SO4 MW: 692.7 Appearance: white to off-white powder Purity: >700 μg/mg
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G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. G418 sulfate (Geneticin) is used routinely in molecular genetics experiments for selecting eukaryotic transformants. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 sulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml.  It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through thenptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells.

G418 longstanding application as a gene selection antibiotic has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

 

 

 

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Synonyms Geneticin
Product # G-1033
CAS # 108321-42-2
Formula C20H40O10N4.2H2SO4
MW 692.7
Appearance white to off-white powder
Purity >700 μg/mg
Solubility Soluble in Water and Aqueous Buffers
Storage Temp +4°C
Use An antibiotic with potent antiparasitic and antiamoebic activity.

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GHS Pictograms
RTECS CB9378500

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msds 1 G-1033 SDS.pdf
Certificate of Analysis 1 G-1033, G1274A.pdf
Certificate of Analysis 2 G-1033, G1274.pdf
Certificate of Analysis 3 G-1033, G1171A.pdf

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Nigericin-induced Impairment of Autophagic Flux in Neuronal Cells Is Inhibited by Overexpression of Bak, Junghyun Lim, Yunsu Lee, et al, J. Biol. Chem., Jul 2012. 287: 23271 - 23282.
7 Interfaces, Teresa A. Murray, Daniel Bertrand, et al, Mol. Pharmacol., Feb 2012. 81: 175 - 188.
Role of MRE11 in Cell Proliferation, Tumor Invasion, and DNA Repair in Breast Cancer, Shyng-Shiou F. Yuan, Ming-Feng Hou, et al, J Natl Cancer Inst, Oct 2012; 104: 1485 - 1502.
Targeting of the Orphan Receptor GPR35 by Pamoic Acid: A Potent Activator of Extracellular Signal-Regulated Kinase and Arrestin 2 with Antinociceptive Activity, Pingwei Zhao et al, Molecular Pharmacology October 2010. 78: 560-568.
PKC and cofilin activation affects peripheral actin reorganization and cell-cell contact in cells expressing integrin 5 but not its tailless mutant. Min-A Oh et al, J Cell Sci., August 1, 2007. 120: 2717-2730
Molecular Mechanism of Constitutive Endocytosis of Acid-Sensing Ion Channel 1a and Its Protective Function in Acidosis-Induced Neuronal Death., Wei-Zheng Zeng et al, The Journal of Neuroscience, 17 April 2013, 33(16):7066-7078.
Endolysosomal Membrane Trafficking Complexes Drive Nutrient-Dependent TORC1 signaling to Control Cell Growth in Saccharomyces cerevisiae. Kingsbury, J. M. et al. Genetics. Apr 2014. 196: 1077-1089.
FUNGAL AND PARASITIC INFECTIONS: Discovery of a Role for Hsp82 in Histoplasma Virulence through a Quantitative Screen for Macrophage Lethality. Edwards, J. A. et al. Infect. Immun. Aug 2011. 79:3348-3357.
VIRUS-CELL INTERACTIONS: Epstein-Barr Virus SM Protein Utilises Cellular Splicing Factor SRp20 To Mediate Alternative Splicing. Verma, D. et al. J. Virol. Nov 2010. 84: 11781-11789.
TRP14 Inhibits Osteoclast Differentiation Via Its Catalytic Activity. Hong, S. et al. Mol. Cell. Biol. Sep 2014. 34: 3515-3524.
PATHOGENESIS AND IMMUNITY: Hepatitis C Virus Entry is Impaired by Claudin-1 Downregulation in Diacylglycerol Acyltransferase-1-Deficient Cells. Sung, P. S. et al. J. Virol. Aug 2014. 88: 9233-9244.
CANCER THERAPY PRECLINICAL: Contribution of OATP1B3 to the Deposition of Sorafenib and Sorafenib-Glucuronide. Zimmerman, E. I. et al. Clin. Cancer Res. March 2013. 19: 1458-1466.
Sphingosine 1-phosphate and human ether-a'-go-go-related gene potassium channels modulate migration in human anaplastic thyroid cancer cells. Asghar, M. Y. et al. Endrocr. Relat. Cancer. Sep 2012. 19:667-680.
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.

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G-418.Sulfate

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