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Human rhinovirus 3C protease (HRV 3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly.
The recombinant form of the HRV3C protease is a restriction grade protease that has robust activity at 4°C and high specific activity.
This HRV 3C Protease is a 47 kDa protein with both GST and His tags so it can be removed by either Ni-chelating or Glutathione (GSH) resin.
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Attached are a few published references from researchers who have used our HRV 3C Protease product. We respectfully thank them for their trust in our product. Inquire for a full reference list.
- A mutation in the mouse Amelx tri-tyrosyl domain results in impaired secretion of amelogenin and phenocopies human X-linked amelogenesis imperfecta, Martin J. Barron, et al, Hum. Mol. Genet., Apr 2010; 19: 1230 - 1247
Preparation: It is recommended to test the HRV3C Protease cleavage with protease-to-target protein ratio of 1:100 (w/w) in the buffer suitable for the target protein at 4oC overnight, with the target protein concentration at or below 1 mg/ml. Under this condition, > 95% of target protein is cleaved in most cases. The efficiency of cleavage varies due to the sequences around the cleavage site, the conformation and the solubility of the target protein. Including reducing agent such as DTT or TCEP in the buffer helps the cleavage. Due to its high specificity, more protease can be used without non-specific cleavage of target proteins. No non-specific cleavage of control cleavage protein is observed at protease-to-control cleavage protein ratio of 1:10.
|Alternative Name||Turbo3C (HRV 3C) Protease, HRV 3C Protease | Cysteine Protease, PreScission Site | A.G. Scientific|
|Appearance||One mg of HRV 3C Protease in 500 l at 2 mg/ml in a storage buffer of 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM TCEP, and 50% glycerol.|
|Preparation||It is recommended to test the HRV3C Protease cleavage with protease-to-target protein ratio of 1:100 (w/w) in the buffer suitable for the target protein at 4oC overnight.|
|Purity||Activity & Specificity: The activity of HRV 3C Protease is tested using a control cleavage protein. 1 mg/ml of HRV 3C Protease has an equivalent of 1 unit activity conventionally used by other suppliers (1 unit of HRV3C protease cleaves >95% target cleavage protein at 4°C for 16 hours).|
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