Hygromycin B, Concentrated Solution

Hygromycin B, Concentrated Solution Product Number: H-1012-CON
Price	Price	Qty	Add to Cart
1x1 mil.	$85.00
5x 1 MU	$340.00

Product Description

Hygromycin B,an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus, is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells.

Use: The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for the selection of resistant cells is 25-1000 µg/ml. Commonly used concentrations for selection are 200 µg/ml for mammalian cells, 20-200 µg/ml for plant cells & bacteria cells and 200-1000 µg/ml for fungi. Your optimum concentration should be tested experimentally.

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Additional Information

Product Number	H-1012-CON
CAS #	CAS [31282-04-9]
Chemical Name	O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
Chemical Formula	C₂₀H₃₇N₃O₁₃
Alternative Name	from Streptomyces hygroscopicus
Appearance	Concentrate Aqueous Solution (Fill Volume 2 ml)
Merck Index	Merck Index: 13.4878
Molecular Weight	M.W. 527.53
Purity	Purity: >80%
RTECS	RTECS: WK2130000
Solubility	Soluble in Water.
Storage Temp	+4°C

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Hygromycin B concentrated solution, CAS [31282-04-9], Hygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. A.G. Scientific