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Hygromycin B,an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus, is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells.
Use: The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for the selection of resistant cells is 25-1000 µg/ml. Commonly used concentrations for selection are 200 µg/ml for mammalian cells, 20-200 µg/ml for plant cells & bacteria cells and 200-1000 µg/ml for fungi. Your optimum concentration should be tested experimentally.
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|CAS #||CAS [31282-04-9]|
|Alternative Name||from Streptomyces hygroscopicus|
|Appearance||Concentrate Aqueous Solution (Fill Volume 2 ml)|
|Merck Index||Merck Index: 13.4878|
|Molecular Weight||M.W. 527.53|
|Solubility||Soluble in Water.|
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