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Hygromycin B Solid, CAS [31282-04-9], Hygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph.  A.G. Scientific

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Hygromycin B, Solid, High Purity, Cell Culture Tested

CAS: 31282-04-9 Formula: C20H37N3O13 MW: 527.53 Appearance: Solid Powder Purity: >95%
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H-1012-SOLID 1 g
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Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species.

Hygromycin's longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system1

http://femsyr.oxfordjournals.org/content/femsyr/15/2/fov004.full.pdf

CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae. 

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Product # H-1012-SOLID
CAS # 31282-04-9
Chemical Name O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
Formula C20H37N3O13
MW 527.53
Appearance Solid Powder
Purity >95%
Solubility Soluble in Water
Boiling Point 897.6±65.0 °C at 760 mmHg
Preparation Working solutions (<2mg>
Storage Temp +4°C
Therapeutic Area Infectious Diseases
Use Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi

Additional Information

Merck Index 13.4878
MDL Number MFCD06795479
ChemACX X1009739-3
InChI InChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
SMILES CN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N

Additional Information

GHS Pictograms
RTECS WK2130000
UN #'S UN 3462
PACKING GROUP I
Handling Warning.Toxic. May be Carcinogenic. May be Teratogenic.

Additional Information

msds 1 H-1012-POWDER_H-1012-SOLID,_Hygromycin_B,_MSDS.pdf
Certificate of Analysis 1 H-1012-SOLID, F1206E.pdf
Certificate of Analysis 2 H-1012-SOLID, F1206D.pdf
Certificate of Analysis 3 H-1012-SOLID, G1045.pdf

Additional Information

Citations The superoxide dismutases of Candida glabrata protect against oxidative damage and are required for lysine biosynthesis, DNA integrity and chronological life survival Marcela Briones-Martin-del-Campo, Emmanuel Orta-Zavalza, Israel Cañas-Villamar, Guadalupe Gutiérrez-Escobedo, Jacqueline Juárez-Cepeda, Karina Robledo-Márquez, Omar Arroyo Helguera, Irene Castaño, and Alejandro De Las Peñas Microbiology, Feb 2015; 161: 300 - 310.\n
Viral Bcl-2 Encoded by the Kaposi's Sarcoma-Associated Herpesvirus Is Vital for Virus Reactivation\nAnastasia Gelgor, Inna Kalt, Shir Bergson, Kevin F. Brulois, Jae U. Jung, and Ronit Sarid\nJ. Virol., May 2015; 89: 5298 - 5307.
Rv2744c Is a PspA Ortholog That Regulates Lipid Droplet Homeostasis and Nonreplicating Persistence in Mycobacterium tuberculosis DJ Bretl, TM Bigley, SS Terhune, TC Zahrt - Journal of Bacteriology, 2014
Systematic exploration of synergistic drug pairs Molecular Systems Biology 7: 544; published online 8 November 2011; doi:10.1038/msb.2011.71\nSubject Categories: bioinformatics; functional genomics
The MprB Extracytoplasmic Domain Negatively Regulates Activation of the Mycobacterium tuberculosis MprAB Two-Component System DJ Bretl, TM Bigley, SS Terhune, TC Zahrt - Journal of Bacteriology, 2014
Reference Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes, Katrin Krumpe, Idan Frumkin, et al. Mol. Biol. Cell. Oct 2012. 23: 3927 - 3935.
Human Telomerase Reverse Transcriptase (hTERT) Is a Novel Target of the Wnt/β-Catenin Pathway in Human Cancer. Yong Zhang, LingLing Toh, et al. J. Biol. Chem. Sep 2012. 287: 32494 - 32511.
A Novel Downstream Regulatory Element Cooperates with the Silencing Machinery to Repress EPA1 Expression in Candida glabrata. Verónica Gallegos-García, Shih-Jung Pan, et al., Genetics, Apr 2012. 190: 1285 - 1297.
Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors. Edith S.A. Hofinger et al, Glycobiology. 2007. 17: 444-453.
Systematic exploration of synergised drug pairs. Cokol, M. et al. Mol Syst Biol. Jul 2014. 7: 544.
The MprB Extracytoplasmic Domain Negatively Regulates Activation of the Mycobacterium tuberculosis MprAB Two-Component System. Bretl, D, J. et al. J. Bacteriol. Jan 2014. 196: 391-406.
Zinc Finger Nuclease-Expressing Baculoviral Vectors Mediate Targeted Genome Integration of Reprogramming Factor Genes to Facilitate the Generation of Human Induced Pluripotent Stem Cells. Phang, R. Z. et al. Stem Cells Trans Med. Dec 2013. 2: 935-945.
Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells. Zhu, H. et al. Nucleic Acid Res. Oct 2013. 41: e180.
Blastomyces dermatidis septins CDC3, CDC10 and CDC12 impact the morphology of yeast and hyphae, but are not required for the phase transition. Marty, A. J. and Gauthier, G. M. Med Mycol. Jan 2013. 51: 93-102.
MprA and DosR Coregulate a Mycobacterium tuberculosis Virulence Operon Encoding Rv1813c and Rv1812c. Bretl, D. J. et al. Infect. Immun. Sept 2012. 80: 3018-3033.
GENE REGULATION: Components of the Rv0081-Rv0088 Locus, Which Encodes a Predicted Formate Hydrogenlyase Complex, Are Coregulated by Rv0081, MprA, and DosR in Mycobacterium tuberculosis. He, H. et al. J. Bacteriol. Oct 2011. 193: 5105-5118.
DNA AND CHROMOSOMES: Phosphorylation of ORC2 Protein Dissociates Origin Recognition Complex from Chromatin and Replication Origins. Lee, K. Y. et al. J. Biol. Chem. Apr 2012. 287: 11891-11898.
MOLECULAR BIOLOGY OF PATHOGENS: PepD Participates in the Myobacterial Stress Response Mediated through MprAB and SigE. Whtie, M. J. et al. J. Bacteriol. Mar 2010. 192: 1498-1510.
Fluorescent Tagging and Cellular Distribution of the Kaposi's Sarcoma-Associated Herpesvirus ORF45 Tegument Protein X Jia, S Shen, Y Lv, Z Zhang, H Guo, H Deng - Journal of Virology, 2016

Additional Information

Hygromycin B, Solid, High Purity, Cell Culture Tested

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