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Product Description
Wheat germ (Triticum aestivum) calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26). Wheat germ calmodulin coupled primarily through SH group (Cys26) to epoxy activated resin. Coupled at 5 mg per mL and useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles. Loading conditions: 100 μM Ca2+, with protease inhibitors and appropriate buffers. Elution conditions: Buffers with 1 mM EDTA or EGTA.
Additional Information
| Product Number | C-1429 |
| Alternative Name | CaMTag™ HD Capture |
| Appearance | 50% slurry in 3 M NaCl, 20mM sodium phosphate buffer, pH, 7.4 and 1 mM EGTA. |
| Purity | Calmodulin Content: 1 mL of resin bound with 5 –5.5 mg of wheat germ calmodulin. |
| Storage Temp | +4°C. |
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All products sold by A.G. Scientific are strictly for Laboratory, Preclinical Research, or Bioprocessing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
