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Product Description
Wheat germ (Triticum aestivum) calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26). In contrast to mammalian CaMs, which lack cysteine, this preparation is ideal for applications requiring both calmodulin activation and residue specific tagging. The single SH moiety at Cys26 can be labeled using a variety of sulfhydryl (SH) reactive protein-modification reagents such as fluorescent maleimide derivatives. Additionally, since wheat germ CaM contains only a single tyrosine residue (Tyr138) verses two tyrosine residues found in mammalian CaM, Tyr138 serves as a mono-specific C-terminal region labeling site. Using Cys26 and Tyr138 allows wheat germ CaM to be independently derivatized at both N- and C-terminal regions within the molecule for structural, proteomic biomarker discovery and protein-protein interaction studies. Wheat germ CaM also has no tryptophan resulting in a distinctive absorbance spectrum when compared to mammalian CaM sequences.
Additional Information
COA Documentation: |
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| Product Number | C-1421 |
| CAS # | Swiss-Prot Entry: P04464 |
| Alternative Name | CaMTag™ DuoSite |
| Appearance | Lyophilized from 5% sucrose, 10 mM sodium phosphate, pH 7.0 at a protein concentration of approximately 1 mg per ml. |
| Purity | Physical Purity: This preparation is greater than 98% by SDS-PAGE stained with Coomassie blue having a single band at approximately 17 kDa; Actual molecular weight is 16,750 Da. Activity: Greater than 35,000 units per mg protein. One unit will stimulate 0.01 unit of 3′:5′-cyclic nucleotide phosphodiesterase in a 3 ml reaction volume at pH 7.5 and 30°C, to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 μM Ca2+. |
| Storage Temp | -20°C. |
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All products sold by A.G. Scientific are strictly for Laboratory, Preclinical Research, or Bioprocessing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
