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siRNA EXPRESSION KITS
Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA molecules, 20-25 nucleotides in length. siRNA plays many roles, but its most notable is in the RNA interference (RNAi) pathway, where it interferes with the expression of specific genes with complementary nucleotide sequence. siRNA also acts in RNAi-related pathways, e.g., as an antiviral mechanism or in shaping the chromatin structure of a genome. The complexity of these pathways is only now being elucidated.
siRNAs and their role in post-transcriptional gene silencing (PTGS) in plants were first discovered by David Baulcombe's group at the Sainsbury Laboratory in Norwich, England and reported in Science in 1999. Thomas Tuschl and colleagues soon reported in Nature that synthetic siRNAs could induce RNAi in mammalian cells. This discovery led to a surge in interest in harnessing RNAi for biomedical research and drug development.
S.-C. Mary Wang, Stephen A. Myers, et al. Nr4a1 siRNA Expression Attenuates -MSH Regulated Gene Expression in 3T3-L1 Adipocytes. Mol. Endocrinol, 2011; 25: 291 - 306.
Kumiko Ui-Tei, Yuki Naito, et al. Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect. Nucleic Acids Res, 2008; 36: 2136 - 2151.
Debashish U. Menon and Victoria H. Meller. A Role for siRNA in X-Chromosome Dosage Compensation in Drosophila melanogaster. Genetics, 2012; 191: 1023 - 1028.
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