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CYAN FLUORESCENT PROTEIN
The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 31.3 kDa monomer with 284 amino acids and an N-terminal His-tag, Ex./Em. = 458/480 nm, extinction coefficient 26000M-1CM-1.
FREE REVIEW ARTICLES
- Two-photon imaging of an inguinal lymph node from a cyan fluorescent protein (CFP) β-actin mouse in which all of the β-actin-expressing cells also express CFP, Cold Spring Harb Protoc, Two-photon imaging of an inguinal lymph node from a cyan fluorescent protein (CFP) β-actin mouse in which all of the β-actin-expressing cells also express CFP, 2010; 2010: pdb.mov80.
- Expression of Gal4-dependent transgenes in cells of the mononuclear phagocyte system labeled with enhanced cyan fluorescent protein using Csf1r-Gal4VP16/UAS-ECFP double-transgenic mice, Dmitry A. Ovchinnikov, Wendy J. M. van Zuylen, et al. Expression of Gal4-dependent transgenes in cells of the mononuclear phagocyte system labeled with enhanced cyan fluorescent protein using Csf1r-Gal4VP16/UAS-ECFP double-transgenic mice. J. Leukoc. Biol, 2008; 83: 430 - 433.
- Specific expression of an oxytocin-enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary, Akiko Katoh, Hiroaki Fujihara, et al. Specific expression of an oxytocin-enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary. J. Endocrinol, 2010; 204: 275 - 285.
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