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ENHANCED GREEN FLUORESCENT PROTEIN
Enhanced green fluorescent protein (EGFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.
FREE REVIEW ARTICLES
- Fluorescently tagged canine adenovirus via modification with protein IX–enhanced green fluorescent protein, Long P. Le, Jing Li, Vladimir V. Ternovoi, et al. Fluorescently tagged canine adenovirus via modification with protein IX–enhanced green fluorescent protein. J. Gen. Virol, 2005; 86: 3201 - 3208.
- Rinderpest virus expressing enhanced green fluorescent protein as a separate transcription unit retains pathogenicity for cattle, Ashley C. Banyard, Jenny Simpson, et al. Rinderpest virus expressing enhanced green fluorescent protein as a separate transcription unit retains pathogenicity for cattle. J. Gen. Virol, 2010; 91: 2918 - 2927.
- Insertion of Enhanced Green Fluorescent Protein in a Hinge Region of Vesicular Stomatitis Virus L Polymerase Protein Creates a Temperature-Sensitive Virus That Displays No Virion-Associated Polymerase Activity In Vitro, John B. Ruedas and Jacques Perrault. Insertion of Enhanced Green Fluorescent Protein in a Hinge Region of Vesicular Stomatitis Virus L Polymerase Protein Creates a Temperature-Sensitive Virus That Displays No Virion-Associated Polymerase Activity In Vitro. J. Virol, 2009; 83: 12241 - 12252.
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