PROTEIN L

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PROTEIN L

Protein L was first isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind immunoglobulins through L chain interaction, from which the name was suggested. It consists of 719 amino acid residues. The molecular weight of Protein L purified from the cell walls of Peptostreptoccus magnus was first estimated as 95kD by SDS-PAGE in the presence of reducing agent 2-mercaptoethanol, while the molecular weight was determined to 76kD by gel chromotography in the presence of 6 M guanidine HCl. Protein L does not contain any interchain disulfide loops, nor does it consist of disulfide-linked subunits.

It was an acidic molecular with a PI of 4.0. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobilins (antibodies), Protein L binds antibodies through light chain interactions. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L.

Despite this wide binding range, Protein L is not a universal antibody-binding protein. Protein L binding is restricted to those antibodies that contain kappa light chains. In humans and mice, most antibody molecules contain kappa (κ) light chains and the remainder have lambda (ι) light chains. Protein L is only effective in binding certain subtypes of kappa light chains. For example, it binds human VκI, VκIII and VκIV subtypes but does not bind the VκII subtype. Binding of mouse immunoglobulins is restricted to those having VκI light chains.
Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain.

Protein L is extremely useful for purification of VLκ-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobilins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.

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Product Number	P-2415
Alternative Name	Proteina/g, protein ag, protein a/g, proteinag, protein-ag, protein-a/g, proteinag, Protein A/G, rProtein A/G, r-protein A/G, recombinant protein A/G, IgG purification, antibody purification, Protein A, Protein G, Protein A&G
Appearance	Lyophilized
Molecular Weight	59.7 kDa
Purity	≥98%
Storage Temp	-20°C

Protein A/G, Recombinant Product Number: P-2415
Price	Price	Qty	Add to Cart
1x1 mg	$85.00
1x5 mg	$235.00
1x25 mg	$895.00
1x100 mg	$2,550.00
1x1 g	$12,500.00

Product Number	P-2411
Alternative Name	Protein A, rProtein A, r-protein A, recombinant protein A, IgG purification, antibody purification, Protein A beads, Protein A sepharose, Protein A Sepharose Beads, proteina, protein-A, protein a, protein/a,
Appearance	Liquid
Molecular Weight	39.21 kDa
Purity	>98% by SDS-PAGE and HPLC
Storage Temp	-20oC or below. Stable, as supplied, for at least 1 year.

Protein A, Recombinant (Liquid Form) Product Number: P-2411
Price	Price	Qty	Add to Cart
1x10 mg	$80.00
1x25 mg	$185.00
1x100 mg	$595.00
1x1 g	$1,595.00

Product Number	P-2412
Alternative Name	proteina, protein-A, protein a, protein/a, Protein A, rProtein A, r-protein A, recombinant protein A, IgG purification, antibody purification, Protein A beads, Protein A sepharose, Protein A Sepharose Beads
Appearance	Lyophilized
Molecular Weight	39.21 kDa
Purity	>98% by SDS-PAGE and HPLC
Storage Temp	-20°C

Protein A, Recombinant (Lyophilized) Product Number: P-2412
Price	Price	Qty	Add to Cart
1x10 mg	$80.00
1x25 mg	$185.00
1x100 mg	$595.00
1x1 g	$1,595.00

Product Number	P-2416
Alternative Name	Proteinagl, protein-agl, protein agl, protein a/g/l, proteina/g/l, proteina/g/l, protein-agl, protein-a/g/l, protein a/g/l, protein/agl, Protein A/G/L, rProtein A/G/L, r-protein A/G/L, recombinant protein A/G/L, IgG purification, antibody purification, Pr
Appearance	Lyophilized
Molecular Weight	101.0 kDa
Purity	>98% by SDS-PAGE and HPLC
Storage Temp	-20°C

Protein A/G/L, Recombinant Product Number: P-2416
Price	Price	Qty	Add to Cart
1x1 mg	$95.00
1x5 mg	$275.00
1x25 mg	$965.00
1x100 mg	$2,950.00
1x1 g	$14,500.00

Product Number	P-2414
Alternative Name	Proteinl, protein L, protein-L, protein/L, Protein L, rProtein L, r-protein L, recombinant protein L, IgG purification, antibody purification, Protein G purification
Appearance	Lyophilized
Molecular Weight	43.2 kDa with 5 IgG binding sites
Purity	≥98%
Storage Temp	Store at -20oC. After reconstitution, aliquot and store at –20oC. Avoid repeated freezing and thawing. Stable, as supplied, for at least 1 year.

Protein L, Recombinant Product Number: P-2414
Price	Price	Qty	Add to Cart
1x1 mg	$105.00
1x5 mg	$325.00
1x10 mg	$525.00
1x100 mg	$3,950.00

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PROTEIN L