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Puromycin CAS 58-58-2   CRISPR Cas9-genome editing

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Puromycin, dihydrochloride

CAS: 58-58-2 Formula: C22H29N7O5 2HCl MW: 544.4 Purity: >98% by HPLC
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Puromycin is a protein inhibitor by inhibiting translation. It is toxic to both procaryotic & eukaryotic cells. Puromycin's main function is used in cell biology as a selective antibiotic agent in cell culture systems for selecting mammalian cell lines, which have been transformed by vectors that express puromycin-N-acetyl-transferase (PAC). 

Puromycin’s longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system1,2.

http://mbio.asm.org/content/6/4/e00861-15.full.pdf+html

* Listed below are a few published articles demonstrating the use of our Puromycin. Inquire for a full list of references.

* Related Sources

Additional Information

Synonyms Stylomycin dihydrochloride, CL 13900 dihydrochloride, CL16536, NSC 3055, P638 dihydrochloride.
Product # P-1033
CAS # 58-58-2
Chemical Name (S) 3'-[[2-Amino-3-(4-methoxyphenyl)-1-oxopropyl]amino]-3'-deoxy-N,N-dimethyladenosine,3'-(a-Amino-p-methoxy-hydocinnamamido)-3'-dexoy-N,N-dimethyladenosine.2HCl
Formula C22H29N7O5 2HCl
MW 544.4
Purity >98% by HPLC
Solubility Soluble in water (50 mg/ml), ethanol (~1 mg/ml), DMSO (~13 mg/ml), DMF (~14 mg/ml), and PBS, pH 72 (~10 mg/ml)
Melting Point 175-177 °C
Boiling Point 752.16 °C
Storage Temp -20°C
Therapeutic Area Infectious Diseases
Use An aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene

Additional Information

Merck Index 14: 7943
MDL Number MFCD00866328
ChemACX X1001900-0
InChI InChI=1S/C22H29N7O5/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32)/t14-,15+,16+,18+,22+/m0/s1
SMILES CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O

Additional Information

RTECS AU7350000
UN #'S UN 2811
PACKING GROUP I

Additional Information

msds 1 P-1033, SDS.pdf
Certificate of Analysis 1 P-1033, G1243.pdf
Certificate of Analysis 2 P-1033, F1219B.pdf
Certificate of Analysis 3 P-1033, F1128R.pdf
E-Brochure Antibiotics Brochure

Additional Information

Additional Information

Puromycin, dihydrochloride

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