![Hygromycin B concentrated solution, CAS [31282-04-9], Hygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. A.G. Scientific](http://www.agscientific.com/media/catalog/product/cache/1/thumbnail/56x/5e06319eda06f020e43594a9c230972d/f/i/file_28.png "Hygromycin B concentrated solution, CAS [31282-04-9], Hygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. A.G. Scientific")
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Product Description
Hygromycin B, an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus, is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells.Use: The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for the selection of resistant cells is 25-1000 µg/ml. Commonly used concentrations for selection are 200 µg/ml for mammalian cells, 20-200 µg/ml for plant cells & bacteria cells and 200-1000 µg/ml for fungi. Your optimum concentration should be tested experimentally.
Interested in our Hygromycin B Blog of Hygromycin's most Frequently Asked Questions (FAQS)?? CLICK HERE.
For a full listing of hygromycin B formulations.CLICK HERE.
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Hygromycin B, an aminoglycosidic antibiotic produced by Streptomyces hygroscopicus, is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. Hygromycin B kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
The resistance gene codes for a kinase that inactivates Hygromycin B through phosphorylation. Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells.
Use: The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for the selection of resistant cells is 25-1000 µg/ml. Commonly used concentrations for selection are 200 µg/ml for mammalian cells, 20-200 µg/ml for plant cells & bacteria cells and 200-1000 µg/ml for fungi. Your optimum concentration should be tested experimentally.
Interested in our Hygromycin B Blog of Hygromycin's most Frequently Asked Questions (FAQS)?? CLICK HERE.
For a full listing of hygromycin B formulations.CLICK HERE.
**********************************
Seeking a unique, hard to source, or cutting edge ANTIBIOTIC? A.G.Scientific's comprehensive Antibiotic E-Booklet contains over 160 novel antibiotics including: Antimicrobial, antifungal, anticancer, immunosuppressive, antivial or antiparasitic agents.
Download your FREE Antibiotic E-booklet NOW. Click here.
Seeking bulk pricing on bulk or multiple vials quantities. All delivered over a set period of time?
Discounts are readily available on all three options. Submit your inquiry NOW. Click here.
************************************
Additional Information
| Product Number | H-1012-CON |
| CAS # | CAS [31282-04-9] |
| Chemical Name | O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine |
| Chemical Formula | C20H37N3O13 |
| Alternative Name | from Streptomyces hygroscopicus |
| Appearance | Concentrate Aqueous Solution (Fill Volume 2 ml) |
| Merck Index | Merck Index: 13.4878 |
| Molecular Weight | M.W. 527.53 |
| Purity | Purity: >80% |
| RTECS | RTECS: WK2130000 |
| Solubility | Soluble in Water. |
| Storage Temp | +4°C |
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![Hygromycin B concentrated solution, CAS [31282-04-9], Hygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. A.G. Scientific](http://www.agscientific.com/media/catalog/product/cache/1/image/5e06319eda06f020e43594a9c230972d/f/i/file_28.png "Hygromycin B concentrated solution, CAS [31282-04-9], Hygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph. A.G. Scientific")
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