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Product Description
Hemocyanin immobilized to aldehyde activated agarose at 5-7 mg KLH per mL. Coupled KLH is then stabilized by additional cross-linking to provide for a leach-free gel and useful to remove IgG’s directed against KLH. Resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Although the resin can be reused after regeneration from 6M guanidine hydrochloride, it is best suited for single use. USE: Approx one mL resin to purify .02 to 0.5 mg of monoclonal antibody; one mL resin to purify >1-5 mg of polyclonal antibody . Loading conditions: Neat serum or acites (or any dilution in a suitable buffer formulation between pH 6-8). Wash buffers containing 0.15 to 0.5 M NaCl should be used to elute unbound antibody.
Additional Information
COA Documentation: |
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| Product Number | H-1188 |
| Appearance | 2x1 mL packed gel. 50% slurry in 3 M NaCl, 20 mM sodium phosphate buffer, pH, 7.4 and 1 mM EGTA |
| Purity | Hemocyanin Content: 1 mL of resin bound with approximately 5 – 7 mg of Keyhole Limpet hemocyanin |
| Storage Temp | +4ºC |
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All products sold by A.G. Scientific are strictly for Laboratory, Preclinical Research, or Bioprocessing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
