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Product Description
High solubility form of Keyhole Limpet Hemocyanin (KLH) activated with succinimidyl 4-(N-maleimidomethyl) cyclohexane-1-caboxylate and formulated to use directly with cysteine containing peptides or haptens that contain a free SH group. KLH is a copper-containing protein isolated from the mollusc Megathura crenulata and a potent immunoactivator commonly used as a carrier in the conjugation of peptides for antibody production. The preparation of KLH used to produce the Maleimide Activated form is chromatographically purified and treated to reduce endotoxins for immunization protocols.
Activation: 150-300 maleimide groups per molecule of KLH (mol. wt. ~ 8 x 106. Each lot of Maleimide Activated KLH is assayed for total number of maleimide groups by titration with 5-((2-(and-3)-S-(acetylmercapto)succinoyl)amino)fluorescein (SAMSA fluorescein).
Activation: 150-300 maleimide groups per molecule of KLH (mol. wt. ~ 8 x 106. Each lot of Maleimide Activated KLH is assayed for total number of maleimide groups by titration with 5-((2-(and-3)-S-(acetylmercapto)succinoyl)amino)fluorescein (SAMSA fluorescein).
Additional Information
COA Documentation: |
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| Product Number | H-1186 |
| Alternative Name | KLH, Agarose Immobilized |
| Merck Index | Swiss-Prot Entry:P81732 |
| Purity | 90% by size exclusion chromatography as a mixture of high and low molecular weight forms; 90% by size exclusion chromatography as a mixture of high and low molecular weight forms; 90% by size exclusion chromatography as a mixture of high and low molecular weight forms; Endotoxin: ≤12 EU/mg; Copper to protein ratio: ≥1.5 x 10 -3 |
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