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Product Description
The ProteomeStore™ Spleen Cytosolic Proteins preparation is a lyophilized formulation of soluble whole spleen proteins in a stabilizing buffer containing protease and phosphatase inhibitors. Suitable for both SDS-PAGE and applications requiring non-denatured proteins. Ideal for proteomic studies which require preservation of naturally occurring protein phosphorylation states. Provides a convenient, stabilized, off-the-shelf proteome reagent which may be used in such applications as profiling studies to discover variations in relative protein abundances, immunocapture of native-state proteins of interest and identifying unique electrophoretic mobility patterns of proteins contained within the spleen proteome. The extract was prepared using a Teflon® pestal homogenizer (Potter-Elvehjem), clarified by centrifugation at 40,000 g for 20 minutes and supernatants immediately frozen for lyophilization. Spleen tissue used in this preparation was obtained from Sprague-Dawley rats, 7-8 weeks old.
Also available: ProteomeStore™ preparations from Sprague Dawley rat brain, heart, kidney, liver, lung; Swiss Webster mouse brain and liver.
Also available: ProteomeStore™ preparations from Sprague Dawley rat brain, heart, kidney, liver, lung; Swiss Webster mouse brain and liver.
Additional Information
| Product Number | C-1614 |
| Appearance | Lyophilized from 5% sucrose in 10 mM HEPES buffer, pH 7.0; 150 mM NaCl; protease inhibitors: 1 mM EDTA, 1 mM EGTA, 1 mM PMSF, 1 mM AEBSF, 5 μg/ml leupeptin, 1 mM benzamidine; phosphatase inhibitors: 50 μM orthovanadate, 100 μM sodium fluoride, 2.5 mM sodi |
| Solubility | Reconstitute in 500 μL H2O. Aliquot and freeze unused material at -20ºC. |
| Storage Temp | -20°C |
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All products sold by A.G. Scientific are strictly for Laboratory, Preclinical Research, or Bioprocessing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
