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Product Description
Mammalian calmodulin isolated from porcine brain is suitable for applications requiring active pure calmodulin. SH GROUP/Protein Ratio: Calmodulin conjugated with SPDP (succinimidyl-3-(2-pyridyldithio)propionate); 2.2 protected SH groups per molecule of calmodulin. Activity: > 35,000 units per mg protein. One unit will stimulate 0.01 unit of 3′:5′-cyclic nucleotide phosphodiesterase in a 3 mL reaction volume at pH 7.5 and 30°C, to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 μM Ca2+. UV Analysis: Absorbance 276 nm of a 1 mg per mL in Milli-Q water, > 0.18. Formulation: Lyophilized from 5% sucrose, 10 mM sodium phosphate, pH 7.0 at a protein concentration of approximately 1 mg per ml.
Additional Information
| Product Number | C-1438 |
| Appearance | White, lyophilized pellet |
| Purity | > 98% by SDS-PAGE stained with Coomassie blue having a single band at approximately 17 kDa; Actual molecular weight is 16,750 Da. |
| Solubility | Solution at a concentration of 1 mg per mL in Milli-Q water, CaM sample is a clear |
| Storage Temp | -20°C. |
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All products sold by A.G. Scientific are strictly for Laboratory, Preclinical Research, or Bioprocessing purposes only. They are not intended for Human Drug, Food Additive, Clinical or Household use.
