CHAPS, High Purity

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CHAPS, High Purity C-1019
  • CAS: 75621-03-3
  • Formula: C32H58N2O7S
  • MW: 614.9
  • Appearance: White Solid
  • Purity: 98%





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CHAPS is a zwitterionic surfactant derived from cholic acid, primarily used to solubilize membrane proteins and other macromolecules. Can be easily removed from gels or protein solutions by dialysis across a membrane. It combines the features of bile salts and N-alkyl sulfobetaine

CHAPS  is used as a nondenaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins, which are often sparingly soluble or insoluble in aqueous solution due to their natively hydrophobic cellular environment. When in salt, CHAPS tends to behave as a weakly charged cation. However, in water or buffer, it behaves as a nonionic surfactant.

CHAPS detergents have low light absorbance in the ultraviolet region of the electromagnetic spectrum, which is useful for laboratory workers monitoring ongoing chemical reactions or protein-protein binding with UV/VIS spectroscopy. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH2 to12. The critical micelle concentration of CHAPS is 6-10mM.

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Additional Information

Product #C-1019
CAS #75621-03-3
Chemical Name3-[3-(Cholamidopropyl)dimethylammonio]-1-propanesulfonate
AppearanceWhite Solid
SolubilitySoluble in DMSO or DMF.
Melting Point157 °C
Storage TempRoom Temp, Warning: Harmful! Hygroscopic!
UseA non-denaturing, zwitterionic detergent for membrane proteins

Additional Information

Merck IndexMerck Index: 13.2052.2001
MDL NumberMFCD00012116

Additional Information

GHS Pictograms
HandlingStore in Tightly Sealed Vial WARNING: HARMFUL! HYGROSCOPIC!

Additional Information

msds 1C-1019, CHAPS, SDS, diamond format.pdf
Certificate of Analysis 1C-1019, J1303.pdf
Certificate of Analysis 2C-1019, J1219.pdf
Certificate of Analysis 3C-1019, J1051A.pdf
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