Coelenterazine h | CAS 50909-86-9 | Fluorescent Probe | AG Scientific, Inc.

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Coelenterazine h

C-2581
  • CAS: 50909-86-9
  • Formula: C26H21N3O2
  • MW: 407.5 Da
  • Appearance: Yellow-orange solid
  • Purity: >98% by HPLC

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Description

Coelenterazine-h (also known as 2-(4-Dehydroxy) coelenterazine) is a specific and highly-sensitive intracellular luminophore that emits blue light when bound to calcium ions. Coelenterazine-h acts as a substrate for luciferases and can be used as a constituent of photoproteins. Approximately 20x greater luminescence than the native form.

 

Applications

• Gene reporter assays
• Live cell assays
• Biochemical assays
• Cell viability fluorescent probe
• Useful for measuring small changes in superoxide and calcium concentrations in cells transfected with apoaequorin cDNA 

 

Specifications

• Emission Maximum: 466 nm
• Relative Luminescence Capacity: 0.82
• Relative Intensity: 10
• Half-Rise Times: 0.4-0.8

 

Additional Information

Synonyms2-Deoxycoelenterazine, CLZN-h, 2-(4-Dehydroxy) coelenterazine, Luciferin (Renilla)
Product #C-2581
CAS #50909-86-9
Chemical Name2-Benzyl-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one
FormulaC26H21N3O2
MW407.5 Da
AppearanceYellow-orange solid
Purity>98% by HPLC
SolubilitySoluble in methanol (10 mg/mL), ethanol (10 mg/mL), and water (slightly soluble). Avoid DMSO as product is unstable in this solvent
Storage TempSolution is susceptible to oxidation by air. For best results, keep solution from light and store at < -70°C under nitrogen or argon. Keep solid at -20 °C or -70 °C and protect from light under nitrogen or argon for long-term storage. Keep calcium free wh
UseCell viability fluorescent probe. Useful for measuring small changes in calcium concentrations.

Additional Information

MDL NumberMFCD00798394
ChemACXX1206765-5
PubChecm ID2762722
InChIInChI=1S/C26H21N3O2/c30-21-13-11-20(12-14-21)24-17-29-25(22(27-24)15-18-7-3-1-4-8-18)28-23(26(29)31)16-19-9-5-2-6-10-19/h1-14,17,27,30H,15-16H2
SMILESC1=CC=C(C=C1)CC2=C3N=C(C(=O)N3C=C(N2)C4=CC=C(C=C4)O)CC5=CC=CC=C5

Additional Information

GHS Pictograms
HandlingAvoid inhalation of vapor or mist. Avoid direct contact with substance.

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