2-8°C
EAH-agarose 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to 4B via epoxy activation.
The free terminal amino group allows for coupling of carboxyl-group containing ligands through amide bond formation by carbodimide-mediated coupling method. Due to the long hydrophilic spacer arm (10-atom), bio-World`s EAH-4B is ideal for immobilization of small molecules which are stable in low pH and contain free carboxyl groups.
Specifications:
- Matrix active groups: -NH2 groups
- Matrix: 4B (agarose beads, 4%)
- Particle size range: 52 – 165 μm
- Matrix activation: Epoxy
- Group to be coupled: -COOH groups
- Chemical linkage of ligand: Stable amide linkage
- Spacer arm: 6-amino caproic acid, 10 atoms
- Ligand density: 7 – 11 μmol amino / ml medium
- Coupling condition: pH 4.5 – 6, Temp: 4 – 25°C
- Storage: 20% ethanol containing 0.9% NaCl
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
