G-418 Sulfate

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G-418 Sulfate

G-1033
  • CAS: 108321-42-2
  • Formula: C20H40O10N4.2H2SO4
  • MW: 692.7
  • Appearance: white to off-white powder
  • Purity: ≥90%

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G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. G418 sulfate (Geneticin) is used routinely in molecular genetics experiments for selecting eukaryotic transformants. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 sulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml.  

It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 has a long standing application as a gene selection antibiotic that has a powerful new role in the genome editing, utilizing the CRISPR/CAS9 system.

 

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SynonymsGeneticin, Glycoside-418, Sulfate; BRN 1669188; Antibiotic G418
Product #G-1033
CAS #108321-42-2
FormulaC20H40O10N4.2H2SO4
MW692.7
Appearancewhite to off-white powder
Purity≥90%
SolubilitySoluble in Water 100mg/ml, forming a colorless solution
Storage TempStore at +4°C. PROTECT FROM LIGHT & MOISTURE! WARNING: HARMFUL! IRRITANT!
UseAn antibiotic with potent antiparasitic and antiamoebic activity.

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GHS Pictograms
RTECSCB9378500
HandlingKeep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

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msds 1G-1033, G-418 . sulfate, SDS, diamond format.pdf
Certificate of Analysis 1G-1033, J1252A.pdf
Certificate of Analysis 2G-1033, J1252.pdf
Certificate of Analysis 3G-1033, J1241.pdf
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CitationsChemokine axes in breast cancer: factors of the tumor microenvironment reshape the CCR7-driven metastatic spread of luminal-A breast tumors
Modulation of HAT activity by the BRCA2 N372H variation is a novel mechanism of paclitaxel resistance in breast cancer cell lines
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VIRUS-CELL INTERACTIONS:\n Epstein-Barr Virus SM Protein Utilizes Cellular Splicing Factor SRp20 To Mediate Alternative Splicing\n Dinesh Verma, Swarna Bais, Melusine Gaillard, and Sankar Swaminathan\n J. Virol., Nov 2010; 84: 11781 - 11789.
Chemokine axes in breast cancer: factors of the tumor microenvironment reshape the CCR7-driven metastatic spread of luminal-A breast tumors
GENE REGULATION, CHROMATIN AND EPIGENETICS:\n A role for YY1 in repression of dominant negative LEF-1 expression in colon cancer\n Noriko N. Yokoyama, Kira T. Pate, Stephanie Sprowl, and Marian L. Waterman\n Nucleic Acids Res., Oct 2010; 38: 6375 - 6388.
HUMAN CANCER BIOLOGY:\n Tumor Suppressor BRCA1 Is Expressed in Prostate Cancer and Controls Insulin-like Growth Factor I Receptor (IGF-IR) Gene Transcription in an Androgen Receptor-Dependent Manner\n Hagit Schayek, Kathy Haugk, Shihua Sun, Lawrence D. True, Stephen R. Plymate, and Haim Werner\n Clin. Cancer Res., Mar 2009; 15: 1558 - 1565.
Voltage- and temperature-dependent activation of TRPV3 channels is potentiated by receptor-mediated PI(4,5)P2 hydrolysis\n Julia F. Doerner, Hanns Hatt, and I. Scott Ramsey\n J. Gen. Physiol., Feb 2011; 137: 271 - 288.
CANCER BIOLOGY:\n The extracellular loop 2 of TM4SF5 inhibits integrin 2 on hepatocytes under collagen type I environment\n Sin-Ae Lee, Young Mee Kim, Tae Kyoung Kwak, Hyeon Jung Kim, Semi Kim, Wonil Ko, Sung-Hoon Kim, Ki Hun Park, Hyun Jeong Kim, Moonjae Cho, and Jung Weon Lee\n Carcinogenesis, Nov 2009; 30: 1872 - 1879.
RESEARCH ARTICLES:\n FHL3 binds MyoD and negatively regulates myotube formation\n Denny L. Cottle, Meagan J. McGrath, Belinda S. Cowling, Imogen D. Coghill, Susan Brown, and Christina A. Mitchell\n J. Cell Sci., Apr 2007; 120: 1423 - 1435.
CELLULAR/MOLECULAR:\n Molecular Mechanism of Constitutive Endocytosis of Acid-Sensing Ion Channel 1a and Its Protective Function in Acidosis-Induced Neuronal Death\n Wei-Zheng Zeng, Di-Shi Liu, Bo Duan, Xing-Lei Song, Xiang Wang, Dong Wei, Wen Jiang, Michael X. Zhu, Yong Li, and Tian-Le Xu\n J. Neurosci., Apr 2013; 33: 7066 - 7078.
CANCER THERAPY: PRECLINICAL:\n Contribution of OATP1B1 and OATP1B3 to the Disposition of Sorafenib and Sorafenib-Glucuronide\n Eric I. Zimmerman, Shuiying Hu, Justin L. Roberts, Alice A. Gibson, Shelley J. Orwick, Lie Li, Alex Sparreboom, and Sharyn D. Baker\n Clin. Cancer Res., Mar 2013; 19: 1458 - 1466.
CELLULAR GENETICS:\n Endolysosomal Membrane Trafficking Complexes Drive Nutrient-Dependent TORC1 Signaling to Control Cell Growth in Saccharomyces cerevisiae\n Joanne M. Kingsbury, Neelam D. Sen, Tatsuya Maeda, Joseph Heitman, and Maria E. Cardenas\n Genetics, Apr 2014; 196: 1077 - 1089.
PRIMARY RESEARCH:\nChemokine axes in breast cancer: factors of the tumor microenvironment reshape the CCR7-driven metastatic spread of luminal-A breast tumors
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Prometaphase arrest-dependent phosphorylation of Bcl-2 family proteins and activation of mitochondrial apoptotic pathway are associated with 17alpha-estradiol-induced apoptosis in human Jurkat T cells
Functional Characterization of pCMAH in the Synthesis of N-Glycolylneuraminic Acid as the Xenoantigenic Determinant in Pig-to-Human Xenotransplantation
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Nucleolar stress enhances lytic reactivation of the Kaposi's sarcoma-associated herpesvirus
Antidepressant drug paroxetine blocks the open pore of Kv3.1 potassium channel
Cyclic mechanical stretch up-regulates hepatoma-derived growth factor expression in cultured rat aortic smooth muscle cells
ReferenceA role for YY1 in repression of dominant negative LEF-1 expression in colon cancer.,Noriko N. Yokoyama et al, Nucleic Acids Research. 2010, 38: 6375
Nigericin-induced Impairment of Autophagic Flux in Neuronal Cells Is Inhibited by Overexpression of Bak, Junghyun Lim, Yunsu Lee, et al, J. Biol. Chem., Jul 2012. 287: 23271 - 23282.
7 Interfaces, Teresa A. Murray, Daniel Bertrand, et al, Mol. Pharmacol., Feb 2012. 81: 175 - 188.
Role of MRE11 in Cell Proliferation, Tumor Invasion, and DNA Repair in Breast Cancer, Shyng-Shiou F. Yuan, Ming-Feng Hou, et al, J Natl Cancer Inst, Oct 2012; 104: 1485 - 1502.
Targeting of the Orphan Receptor GPR35 by Pamoic Acid: A Potent Activator of Extracellular Signal-Regulated Kinase and Arrestin 2 with Antinociceptive Activity, Pingwei Zhao et al, Molecular Pharmacology October 2010. 78: 560-568.
PKC and cofilin activation affects peripheral actin reorganization and cell-cell contact in cells expressing integrin 5 but not its tailless mutant. Min-A Oh et al, J Cell Sci., August 1, 2007. 120: 2717-2730
Molecular Mechanism of Constitutive Endocytosis of Acid-Sensing Ion Channel 1a and Its Protective Function in Acidosis-Induced Neuronal Death., Wei-Zheng Zeng et al, The Journal of Neuroscience, 17 April 2013, 33(16):7066-7078.
Endolysosomal Membrane Trafficking Complexes Drive Nutrient-Dependent TORC1 signaling to Control Cell Growth in Saccharomyces cerevisiae. Kingsbury, J. M. et al. Genetics. Apr 2014. 196: 1077-1089.
FUNGAL AND PARASITIC INFECTIONS: Discovery of a Role for Hsp82 in Histoplasma Virulence through a Quantitative Screen for Macrophage Lethality. Edwards, J. A. et al. Infect. Immun. Aug 2011. 79:3348-3357.
VIRUS-CELL INTERACTIONS: Epstein-Barr Virus SM Protein Utilises Cellular Splicing Factor SRp20 To Mediate Alternative Splicing. Verma, D. et al. J. Virol. Nov 2010. 84: 11781-11789.
TRP14 Inhibits Osteoclast Differentiation Via Its Catalytic Activity. Hong, S. et al. Mol. Cell. Biol. Sep 2014. 34: 3515-3524.
PATHOGENESIS AND IMMUNITY: Hepatitis C Virus Entry is Impaired by Claudin-1 Downregulation in Diacylglycerol Acyltransferase-1-Deficient Cells. Sung, P. S. et al. J. Virol. Aug 2014. 88: 9233-9244.
CANCER THERAPY PRECLINICAL: Contribution of OATP1B3 to the Deposition of Sorafenib and Sorafenib-Glucuronide. Zimmerman, E. I. et al. Clin. Cancer Res. March 2013. 19: 1458-1466.
Sphingosine 1-phosphate and human ether-a'-go-go-related gene potassium channels modulate migration in human anaplastic thyroid cancer cells. Asghar, M. Y. et al. Endrocr. Relat. Cancer. Sep 2012. 19:667-680.
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.
RESEARCH ARTICLES:\n Conserved C-Terminal Motifs Required for Avirulence and Suppression of Cell Death by Phytophthora sojae effector Avr1b\n Daolong Dou, Shiv D. Kale, Xinle Wang, Yubo Chen, Qunqing Wang, Xia Wang, Rays H.Y. Jiang, Felipe D. Arredondo, Ryan G. Anderson, Poulami B. Thakur, John M. McDowell, Yuanchao Wang, and Brett M. Tyler\n PLANT CELL, Apr 2008; 20: 1118 - 1133.

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