G-418 Sulfate Solid | Gene Selection Antibiotic | AG Scientific, Inc.

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G-418 Sulfate

G-1033
  • CAS: 108321-42-2
  • Formula: C20H40O10N4 • 2H2SO4
  • MW: 692.7 Da
  • Appearance: White to off-white powder
  • Purity: ≥92%

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G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. G418 sulfate (Geneticin) is used routinely in molecular genetics experiments for selecting eukaryotic transformants. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 sulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml.  

It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 has a long-standing application as a gene selection antibiotic that has a powerful new role in genome editing, utilizing the CRISPR/CAS9 system.

 

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SynonymsGeneticin, Glycoside-418 Sulfate, BRN 1669188, Antibiotic G418
Product #G-1033
CAS #108321-42-2
Chemical Name(2R,3S,4R,5R,6S)-5-Amino-6-[(1R,2S,3S,4R,6S)-4,6-diamino-3-[(2R,3R,4R,5R)-3,5-dihydroxy-5-methyl-4-methylaminooxan-2-yl]oxy-2-hydroxycyclohexyl]oxy- 2-(1-hydroxyethyl)oxane-3,4-diol
FormulaC20H40O10N4 • 2H2SO4
MW692.7 Da
AppearanceWhite to off-white powder
Purity≥92%
SolubilitySoluble in water: 100mg/ml, colorless solution
Storage TempStore at +4°C
UseAn antibiotic with potent antiparasitic and antiamoebic activity

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GHS Pictograms
RTECSCB9378500
HandlingKeep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Protect from light and moisture! Warning: Harmful! Irritant!

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msds 1G-1033, G-418 . sulfate, SDS, diamond format.pdf
Certificate of Analysis 1G-1033, J1252A.pdf
Certificate of Analysis 2G-1033, J1252.pdf
Certificate of Analysis 3G-1033, J1241.pdf
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