*AG Scientific's G-418 sulfate solution is a sterile filterate solution.*
G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 disulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml. It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.
Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.
Purity data is for a concentration of 50mg/ml active.
|Synonyms||Geneticin solution, Glycoside-418 solution, Antibiotic G418 solution|
|Chemical Name||(2R,3S,4R,5R,6S)-5-Amino-6-[(1R,2S,3S,4R,6S)-4,6-diamino-3-[(2R,3R,4R,5R)-3,5-dihydroxy-5-methyl-4-methylaminooxan-2-yl]oxy-2-hydroxycyclohexyl]oxy- 2-(1-hydroxyethyl)oxane-3,4-diol|
|Formula||C20H40O10N4 • 2H2SO4|
|Appearance||Clear and colorless solution|
|Storage Temp||Store at 4°C|
|Use||An antibiotic with potent antiparasitic and antiamoebic activity|
|Handling||Protect from light!|
|msds 1||G-1033, G-418 . sulfate, SDS, diamond format.pdf|
|Certificate of Analysis 1||G-1035, H1291.pdf|
|Certificate of Analysis 2||G-1035, G1274.pdf|
|Certificate of Analysis 3||G-1035, F1294E.pdf|
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