G-418 Sulfate Sterile-Filtered Solution

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G-418 Sulfate Sterile-Filtered Solution

G-1035
  • CAS: 108321-42-2
  • Formula: C20H40O10N4.2H2SO4
  • MW: 692.7
  • Appearance: Clear and colorless solution
  • Purity: ≥90% (TLC)

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*AG Scientific's G-418 sulfate solution is a sterile filterate solution.*

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 disulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml.  It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.

Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

 Purity data is for a concentration of 50mg/ml active.

 

Additional Information

SynonymsGeneticin, Glycoside-418, Antibiotic G418
Product #G-1035
CAS #108321-42-2
FormulaC20H40O10N4.2H2SO4
MW692.7
AppearanceClear and colorless solution
Purity≥90% (TLC)
Storage TempStore at -4°C. PROTECT FROM LIGHT

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GHS Pictograms
RTECSCB9378500

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Certificate of Analysis 1G-1035, H1291.pdf
Certificate of Analysis 2G-1035, G1274.pdf
Certificate of Analysis 3G-1035, F1294E.pdf
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CitationsRoles of unphosphorylated ISGF3 in HCV infection and interferon responsiveness\nPil Soo Sung, HyeonJoo Cheon, Chung Hwan Cho, Seon-Hui Hong, Do Youn Park, \nHyung-Il Seo, Su-Hyung Park, Seung Kew Yoon, George R. Stark, and Eui-Cheol Shin\nPNAS, Aug 2015; 112: 10443 - 10448.
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ReferenceNigericin-induced Impairment of Autophagic Flux in Neuronal Cells Is Inhibited by Overexpression of Bak, Junghyun Lim, Yunsu Lee, et al, J. Biol. Chem., Jul 2012; 287: 23271 - 23282.
Alpha 7 beta 2 Nicotinic Acetylcholine Receptors Assemble, Function, and Are Activated Primarily via Their alpha7 - alpha7 Interfaces, Teresa A. Murray, Daniel Bertrand, Roger L. Papke, Andrew A. George, Rigo Pantoja, Rahul Srinivasan, Qiang Liu, Jie Wu, Paul Whiteaker, Henry A. Lester and Ronald J. Lukas
Role of MRE11 in Cell Proliferation, Tumor Invasion, and DNA Repair in Breast Cancer, Shyng-Shiou F. Yuan, Ming-Feng Hou, et al, J Natl Cancer Inst, Oct 2012; 104: 1485 - 1502.
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae

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