*AG Scientific's G-418 sulfate solution is a sterile filterate solution.*
G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 disulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml. It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.
Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.
Purity data is for a concentration of 50mg/ml active.
|Synonyms||Geneticin, Glycoside-418, Antibiotic G418|
|Appearance||Clear and colorless solution|
|Storage Temp||Store at -4°C. PROTECT FROM LIGHT|
|Certificate of Analysis 1||G-1035, H1291.pdf|
|Certificate of Analysis 2||G-1035, G1274.pdf|
|Certificate of Analysis 3||G-1035, F1294E.pdf|
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