G-418 Sulfate Solution | CAS 108321-42-2 | Antibiotics | AG Scientific, Inc.

More Views

G-418 Sulfate Solution, Sterile-Filtered

G-1035
  • CAS: 108321-42-2
  • Formula: C20H40O10N4 • 2H2SO4
  • MW: 692.7 Da
  • Appearance: Clear and colorless solution
  • Purity: ≥90% (TLC)

2

1

0

Hazardous
Add to Cart Bulk Inquiry

*AG Scientific's G-418 sulfate solution is a sterile filterate solution.*

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. G418 disulfate is freely soluble in water. Bacteria and algae require 5 µg/ml or less while animal cells may require 300-500 µg/ml.  It functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.

Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

 Purity data is for a concentration of 50mg/ml active.

 

Additional Information

SynonymsGeneticin solution, Glycoside-418 solution, Antibiotic G418 solution
Product #G-1035
CAS #108321-42-2
Chemical Name(2R,3S,4R,5R,6S)-5-Amino-6-[(1R,2S,3S,4R,6S)-4,6-diamino-3-[(2R,3R,4R,5R)-3,5-dihydroxy-5-methyl-4-methylaminooxan-2-yl]oxy-2-hydroxycyclohexyl]oxy- 2-(1-hydroxyethyl)oxane-3,4-diol
FormulaC20H40O10N4 • 2H2SO4
MW692.7 Da
AppearanceClear and colorless solution
Purity≥90% (TLC)
Storage TempStore at 4°C
UseAn antibiotic with potent antiparasitic and antiamoebic activity

Additional Information

Additional Information

GHS Pictograms
RTECSCB9378500
HandlingProtect from light!

Additional Information

msds 1G-1033, G-418 . sulfate, SDS, diamond format.pdf
Certificate of Analysis 1G-1035, H1291.pdf
Certificate of Analysis 2G-1035, G1274.pdf
Certificate of Analysis 3G-1035, F1294E.pdf
Close pop-up box

Personal Information

* Required Fields

Additional Information

CitationsSung, Pil Soo, et al. "Roles of unphosphorylated ISGF3 in HCV infection and interferon responsiveness." Proceedings of the National Academy of Sciences 112.33 (2015): 10443-10448.
Deliu, Elena, et al. "The lysophosphatidylinositol receptor GPR55 modulates pain perception in the periaqueduactal grey." Molecular pharmacology (2015): mol-115.
Pandhare, Akash, et al. "The antidepressant bupropion is a negative allosteric modulator of serotonin type 3A receptors." Neuropharmacology 113 (2017): 89-99.
Izraely, Sivan, et al. "ANGPTL4 promotes the progression of cutaneous melanoma to brain metastasis." Oncotarget 8.44 (2017): 75778.
Zhang, Jin, et al. "Disruption of the cell wall integrity gene ECM33 results in improved fermentation by wine yeast." Metabolic engineering 45 (2018): 255-264.
Seo-hee, Nam. Novel Functions of Lysyl-TRNA Synthetase in Invasive Dissemination of Colon Cancer Cells in 3D Microenvironment. Edited by Lee Jung Won, Seoul National University Graduate School, 2017, Novel Functions of Lysyl-TRNA Synthetase in Invasive Dissemination of Colon Cancer Cells in 3D Microenvironment.
Lee, Hyunjae, et al. "A microfluidic platform for quantitative analysis of cancer angiogenesis and intravasation." Biomicrofluidics 8.5 (2014): 054102.
Kumari, S. Sindhu, et al. "Spatial expression of aquaporin 5 in mammalian cornea and lens, and regulation of its localization by phosphokinase A." Molecular vision 18 (2012): 957.
Moon, YeonHee, et al. "Effect of CD133 overexpression on the epithelial-to-mesenchymal transition in oral cancer cell lines." Clinical & experimental metastasis 33.5 (2016): 487-496.
Paproski, Robert J., et al. "Multi-wavelength photoacoustic imaging of inducible tyrosinase reporter gene expression in xenograft tumors." Scientific reports 4 (2014): 5329.
Ng, Yee-Wen, and Yee-How Say. "Palmitic acid induces neurotoxicity and gliatoxicity in SH-SY5Y human neuroblastoma and T98G human glioblastoma cells." PeerJ 6 (2018): e4696.
Lin, Haiyue, et al. "Enhancement of 5-HT2A receptor function and blockade of Kv1. 5 by MK801 and ketamine: implications for PCP derivative-induced disease models." Experimental & molecular medicine 50.4 (2018).
Gelgor, Anastasia, et al. "Nucleolar stress enhances lytic reactivation of the Kaposis sarcoma-associated herpesvirus." Oncotarget 9.17 (2018): 13822.
Lee, Hyang Mi, et al. "Antidepressant drug paroxetine blocks the open pore of Kv3. 1 potassium channel." The Korean Journal of Physiology & Pharmacology 22.1 (2018): 71-80.
Kao, Ying-Hsien, et al. "Cyclic mechanical stretch up-regulates hepatoma-derived growth factor expression in cultured rat aortic smooth muscle cells." Bioscience reports (2018): BSR20171398.
Bergson, Shir, et al. "Fluorescent Tagging and Cellular Distribution of the Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Open Reading Frame 45 (ORF45) Tegument Protein." Journal of virology (2014): JVI-01091.
Yokoyama, Noriko N., et al. "A role for YY1 in repression of dominant negative LEF-1 expression in colon cancer." Nucleic acids research 38.19 (2010): 6375-6388.
ReferenceNigericin-induced Impairment of Autophagic Flux in Neuronal Cells Is Inhibited by Overexpression of Bak, Junghyun Lim, Yunsu Lee, et al, J. Biol. Chem., Jul 2012; 287: 23271 - 23282.
Alpha 7 beta 2 Nicotinic Acetylcholine Receptors Assemble, Function, and Are Activated Primarily via Their alpha7 - alpha7 Interfaces, Teresa A. Murray, Daniel Bertrand, Roger L. Papke, Andrew A. George, Rigo Pantoja, Rahul Srinivasan, Qiang Liu, Jie Wu, Paul Whiteaker, Henry A. Lester and Ronald J. Lukas
Role of MRE11 in Cell Proliferation, Tumor Invasion, and DNA Repair in Breast Cancer, Shyng-Shiou F. Yuan, Ming-Feng Hou, et al, J Natl Cancer Inst, Oct 2012; 104: 1485 - 1502.
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae

Additional Information

No Items Found

Newsletter

Got Questions? We have Answers!

Our team of experts are standing by to help you in every possible way.

Real People, Real Time, Rapid Response

Chip Lindgren

Founder/CEO

Ken Smith

Operations

Kyle Anderson

Procurement & Business Development

Skyler King

Customer Service

 

Nicole Tran

Customer Service

 

Casey Kennedy

Marketing

 

 

 

 

 

BULK ENQUIRY FORM

* Required Fields

*
*
*
*
*
*Bulk OEM Multiple Catalog Custom Other
Prod#CAS#*PRODUCT NAMEGrade*Size*QtyTime Frame

Custom magento captcha module from Outsource Online