Guanidine Hydrochloride

G-2589
  • CAS: 50-01-1
  • Formula: CH5N3•HCl
  • MW: 95.53
  • Appearance: Crystalline powder
  • Purity: ≥99%

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Guanidine Hydrochloride is a powerful, chaotropic agent which is widely used for purification of proteins and nucleic acids. Guanidine Hydrochloride is useful for denaturation and refolding of proteins as well as in the recovery of periplasmic proteins and isolation of RNA. At higher concentrations, this strong denaturant has been reported to solubilize denatured insoluble proteins such as inclusion bodies. When utilized at lower concentrations, Guanidine Hydrochloride is noted to be useful in prompting the refolding of denatured proteins. Guanidine Hydrochloride is also been reported to allow return of enzymatic activity in protocols using Guanidine Hydrochloride as a first step. Guanidine Hydrochloride is an inhibitor of RNase.

Additional Information

Product #G-2589
CAS #50-01-1
FormulaCH5N3•HCl
MW95.53
AppearanceCrystalline powder
Purity≥99%
SolubilitySoluble in water (6 M) at 20° C, ethanol (300 g/l), and alcohol.
Melting Point185-189° C
Storage TempStore at room temperature

Additional Information

SMILESC(=N)(N)N.Cl

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Hazard ImageYes

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