Hygromycin B in DI Water

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Hygromycin B in DI Water

H-1012-DI
  • CAS: 31282-04-9
  • Formula: C20H37N3O13
  • MW: 527.52
  • Appearance: Clear and yellowish solution (Hygromycin B in DI water)
  • Purity: ≥90%

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* The Concentration of Hygromycin B in DI water is 100mg/ml

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

 

* ATTENTION! International shipping of this material can be subject to an additional hazardous shipping fee. An AG Scientific customer service representative will contact you to confirm cost prior to shipping your international order. 

Additional Information

Product #H-1012-DI
CAS #31282-04-9
Chemical NameO-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
FormulaC20H37N3O13
MW527.52
AppearanceClear and yellowish solution (Hygromycin B in DI water)
Purity≥90%
Storage TempStore at +4°C. Protect from light.
Therapeutic AreaInfectious Diseases
UseHygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi

Additional Information

Merck Index13.4878
MDL NumberMFCD06795479
ChemACXX1009739-3
InChIInChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
SMILESCN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N

Additional Information

GHS Pictograms
RTECSWK2130000
UN #'SUN 3462
PACKING GROUPI
HandlingWarning.Toxic. May be Carcinogenic. May be Teratogenic. Use only in area provided with appropriate exhaust ventilation .

Additional Information

msds 1H-1012, Hygromycin B in DI , SDS, diamond format.pdf
Certificate of Analysis 1H-1012-DI, J1258.pdf
Certificate of Analysis 2H-1012-DI, H1079B.pdf
Certificate of Analysis 3H-1012-DI, H1079A.pdf
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