Hygromycin B, High Purity In PBS Buffer

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Hygromycin B, High Purity In PBS Buffer

H-1012-PBS
  • CAS: 31282-04-9
  • Formula: C20H37N3O13
  • MW: 527.52
  • Appearance: Clear and yellowish solution (Hygromycin B in PBS buffer)
  • Purity: ≥90%

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* The concentration of Hygromycin B in PBS buffer is 50 mg/ml 

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic, has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.

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Additional Information

Product #H-1012-PBS
CAS #31282-04-9
Chemical NameO-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
FormulaC20H37N3O13
MW527.52
AppearanceClear and yellowish solution (Hygromycin B in PBS buffer)
Purity≥90%
SolubilitySoluble in Water
PreparationWorking solutions (<2mg>
Storage TempStore at +4°C. Protect from light.
Therapeutic AreaInfectious Diseases
UseHygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi

Additional Information

Merck Index13.4878
MDL NumberMFCD06795479
ChemACXX1009739-3
InChIInChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
SMILESCN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N

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GHS Pictograms
RTECS WK2130000
PACKING GROUPI

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msds 1H-1012-PBS, SDS, EU.pdf
Certificate of Analysis 1H-1012, Hygromycin B in PBS , SDS, diamond format.pdf
Certificate of Analysis 2H-1012-PBS, H1079B.pdf
Certificate of Analysis 3H-1012-PBS, H1079A.pdf
E-Brochure Fermentation
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CitationsMETHODS ONLINE:\n Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors\n Chrishan J. A. Ramachandra, Mohammad Shahbazi, Timothy W. X. Kwang, Yukti Choudhury, Xiao Ying Bak, Jing Yang, and Shu Wang\n Nucleic Acids Res., Sep 2011; 39: e107.
RETINAL CELL BIOLOGY:\n CaMKIIB Mediates a Survival Response in Retinal Ganglion Cells Subjected to a Glutamate Stimulus\n Wei Fan, Xiaohong Li, and Nigel G. F. Cooper\n Invest. Ophthalmol. Vis. Sci., Aug 2007; 48: 3854 - 3863.
Engineering CHO cells with an oncogenic KIT improves cells growth, resilience to stress, and productivity
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Efficiency to discovery transgenic loci in GM rice using next generation sequencing whole genome re-sequencing
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Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing/K Strain in Mice
The use of light in cancer immunotherapy
CRISPR/Cas9-Mediated Gene Disruption Reveals the Importance of Zinc Metabolism for Fitness of the Dimorphic Fungal Pathogen Blastomyces dermatitidis
CELLULASE 6 and MANNANASE 7 affect cell differentiation and silique dehiscence
ReferenceErgosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes, Katrin Krumpe, Idan Frumkin, et al. Mol. Biol. Cell. Oct 2012. 23: 3927 - 3935.
Human Telomerase Reverse Transcriptase (hTERT) Is a Novel Target of the Wnt/beta-Catenin Pathway in Human Cancer
A Novel Downstream Regulatory Element Cooperates with the Silencing Machinery to Repress EPA1 Expression in Candida glabrata
Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors. Edith S.A. Hofinger et al. Glycobiology. 2007. 17: 444-453.
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.
GENE REGULATION:\n Components of the Rv0081-Rv0088 Locus, Which Encodes a Predicted Formate Hydrogenlyase Complex, Are Coregulated by Rv0081, MprA, and DosR in Mycobacterium tuberculosis\n Hongjun He, Daniel J. Bretl, Renee M. Penoske, David M. Anderson, and Thomas C. Zahrt\n J. Bacteriol., Oct 2011; 193: 5105 - 5118.

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