Hygromycin B in PBS Buffer | CAS 31282-04-9 | AG Scientific, Inc.

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Hygromycin B, High Purity In PBS Buffer

  • CAS: 31282-04-9
  • Formula: C20H37N3O13
  • MW: 527.52 Da
  • Appearance: Clear, yellowish solution
  • Purity: ≥90%




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* The concentration of Hygromycin B in PBS buffer is 50 mg/ml 

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.

Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic, has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.

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Additional Information

SynonymsHygrovetine, Hygrovectine, D-streptamine
Product #H-1012-PBS
CAS #31282-04-9
Chemical NameO-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
MW527.52 Da
AppearanceClear, yellowish solution
SolubilitySoluble in Water
PreparationWorking solutions (<2mg>
Storage TempStore at +4°C
Therapeutic AreaInfectious diseases
UseHygromycin B is an aminoglycoside antibiotic that kills bacteria and fungi.

Additional Information

Merck Index13.4878
MDL NumberMFCD06795479

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GHS Pictograms
RTECS WK2130000
HandlingProtect from light

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msds 1H-1012, Hygromycin B in PBS , SDS, diamond format.pdf
Certificate of Analysis 1H-1012-PBS, J1047.pdf
Certificate of Analysis 2H-1012-PBS, H1079B.pdf
Certificate of Analysis 3H-1012-PBS, H1079A.pdf
E-Brochure Fermentation
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CitationsRamachandra, Chrishan JA, et al. "Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors." Nucleic acids research 39.16 (2011): e107-e107.
Fan, Wei, Xiaohong Li, and Nigel GF Cooper. "CaMKIIaB mediates a survival response in retinal ganglion cells subjected to a glutamate stimulus." Investigative ophthalmology & visual science 48.8 (2007): 3854-3863.
Mahameed, Mohamed, and Boaz Tirosh. "Engineering CHO cells with an oncogenic KIT improves cells growth, resilience to stress, and productivity." Biotechnology and bioengineering 114.11 (2017): 2560-2570.
Kou, Yanjun, et al. "Structure-function analyses of the Pth11 receptor reveal an important role for CFEM motif and redox regulation in rice blast." New Phytologist 214.1 (2017): 330-342.
Park, Doori, et al. "Efficiency to discovery transgenic loci in GM rice using next generation sequencing whole genome re-sequencing." Genomics & informatics 13.3 (2015): 81.
Qu, Baiyan, et al. "Droplet electroporation in microfluidics for efficient cell transformation with or without cell wall removal." Lab on a Chip 12.21 (2012): 4483-4488.
Kim, Ahreum, et al. "Protective vaccine efficacy of the complete form of PPE39 protein from Mycobacterium tuberculosis Beijing/K strain in mice." Clinical and Vaccine Immunology (2017): CVI-00219.
Kleinovink, E. W. J. The use of light in cancer immunotherapy. Diss. 2018.
Kujoth, Gregory C., et al. "CRISPR/Cas9-Mediated Gene Disruption Reveals the Importance of Zinc Metabolism for Fitness of the Dimorphic Fungal Pathogen Blastomyces dermatitidis." mBio 9.2 (2018): e00412-18.
He, Hanjun, et al. "CELLULASE 6 and MANNANASE 7 affect cell differentiation and silique dehiscence." Plant physiology (2018): pp-01494.
Bergson, Shir, et al. "Fluorescent Tagging and Cellular Distribution of the Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Open Reading Frame 45 (ORF45) Tegument Protein." Journal of virology (2014): JVI-01091.
Cokol, Murat, et al. "Systematic exploration of synergistic drug pairs." Molecular systems biology 7.1 (2011): 544.
ReferenceErgosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes, Katrin Krumpe, Idan Frumkin, et al. Mol. Biol. Cell. Oct 2012. 23: 3927 - 3935.
Human Telomerase Reverse Transcriptase (hTERT) Is a Novel Target of the Wnt/beta-Catenin Pathway in Human Cancer
A Novel Downstream Regulatory Element Cooperates with the Silencing Machinery to Repress EPA1 Expression in Candida glabrata
Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors. Edith S.A. Hofinger et al. Glycobiology. 2007. 17: 444-453.
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.
GENE REGULATION:\n Components of the Rv0081-Rv0088 Locus, Which Encodes a Predicted Formate Hydrogenlyase Complex, Are Coregulated by Rv0081, MprA, and DosR in Mycobacterium tuberculosis\n Hongjun He, Daniel J. Bretl, Renee M. Penoske, David M. Anderson, and Thomas C. Zahrt\n J. Bacteriol., Oct 2011; 193: 5105 - 5118.

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