Hygromycin B Solid, CAS [31282-04-9], Hygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells transfected with the hygromycin resistance gene, hph.  A.G. Scientific

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Hygromycin B Solid, High Purity, Cell Culture Tested

H-1012-SOLID
  • CAS: 31282-04-9
  • Formula: C20H37N3O13
  • MW: 527.5
  • Appearance: Cream colored powder
  • Purity: ≥90%

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Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for the selection of hygromycin resistance gene transformed cells. Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in-vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.

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Product #H-1012-SOLID
CAS #31282-04-9
Chemical NameO-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
FormulaC20H37N3O13
MW527.5
AppearanceCream colored powder
Purity≥90%
SolubilitySoluble in Water (50 mg/ml H2O). Clear and light tan solution.
Preparation Working solutions (<2mg>
Storage TempStore at +4°C. PROTECT FROM MOISTURE!
Therapeutic AreaInfectious Diseases
UseHygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria and fungi.

Additional Information

Merck Index13.4878
MDL NumberMFCD06795479
ChemACXX1009739-3
InChIInChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
SMILESCN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)OC4(O3)[C@@H]([C@@H]([C@H]([C@H](O4)[C@H](CO)N)O)O)O)O)N

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GHS Pictograms
RTECSWK2130000
UN #'SUN 3462
PACKING GROUPI
HandlingWarning.Toxic. May be Carcinogenic. May be Teratogenic.

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msds 1H-1012, Hygromycin B, SDS, diamond format.pdf
Certificate of Analysis 1H-1012-SOLID, J1258.pdf
Certificate of Analysis 2H-1012-SOLID, J1047.pdf
Certificate of Analysis 3H-1012-SOLID, H1079A.pdf
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CitationsThe superoxide dismutases of Candida glabrata protect against oxidative damage and are required for lysine biosynthesis, DNA integrity and chronological life survival.Castano1, Alejandro De Las Penas1
Viral Bcl-2 Encoded by the Kaposi's Sarcoma-Associated Herpesvirus Is Vital for Virus Reactivation
Rv2744c Is a PspA Ortholog That Regulates Lipid Droplet Homeostasis and Nonreplicating Persistence in Mycobacterium tuberculosis DJ Bretl, TM Bigley, SS Terhune, TC Zahrt - Journal of Bacteriology, 2014
Systematic exploration of synergistic drug pairs Molecular Systems Biology 7: 544; published online 8 November 2011; doi:10.1038/msb.2011.71\nSubject Categories: bioinformatics; functional genomics
The MprB Extracytoplasmic Domain Negatively Regulates Activation of the Mycobacterium tuberculosis MprAB Two-Component System DJ Bretl, TM Bigley, SS Terhune, TC Zahrt - Journal of Bacteriology, 2014
Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators
BIOSYNTHESIS AND BIODEGRADATION:\n Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes\n Katrin Krumpe, Idan Frumkin, Yonatan Herzig, Nitzan Rimon, Cagakan Ozbalci, Britta Brugger, Doron Rapaport, and Maya Schuldiner\n Mol. Biol. Cell, Oct 2012; 23: 3927 - 3935.
GENE EXPRESSION:\n A Novel Downstream Regulatory Element Cooperates with the Silencing Machinery to Repress EPA1 Expression in Candida glabrata\n Veronica Gallegos-Garcia, Shih-Jung Pan, Jacqueline Juarez-Cepeda, Candy Y. Ramirez-Zavaleta, Marcela Briones Martin-del-Campo, Veronica Martinez-Jimenez, Irene Castano, Brendan Cormack, and Alejandro De Las Penas\n Genetics, Apr 2012; 190: 1285 - 1297.
The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets\n Oludotun Adeyo, Patrick J. Horn, SungKyung Lee, Derk D. Binns, Anita Chandrahas, Kent D. Chapman, and Joel M. Goodman\n J. Cell Biol., Mar 2011; 192: 1043 - 1055.
Engineering CHO cells with an oncogenic KIT improves cells growth, resilience to stress, and productivity
Structure-function analyses of the Pth11 receptor reveal an important role for CFEM motif and redox regulation in rice blast
Efficiency to discovery transgenic loci in GM rice using next generation sequencing whole genome re-sequencing
Droplet electroporation in microfluidics for efficient cell transformation with or without cell wall removal
Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing/K Strain in Mice
The use of light in cancer immunotherapy
CRISPR/Cas9-Mediated Gene Disruption Reveals the Importance of Zinc Metabolism for Fitness of the Dimorphic Fungal Pathogen Blastomyces dermatitidis
CELLULASE 6 and MANNANASE 7 affect cell differentiation and silique dehiscence
ReferenceErgosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes
Human Telomerase Reverse Transcriptase (hTERT) Is a Novel Target of the Wnt/beta-Catenin Pathway in Human Cancer
A Novel Downstream Regulatory Element Cooperates with the Silencing Machinery to Repress EPA1 Expression in Candida glabrata
Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors. Edith S.A. Hofinger et al, Glycobiology. 2007. 17: 444-453.
Systematic exploration of synergised drug pairs. Cokol, M. et al. Mol Syst Biol. Jul 2014. 7: 544.
The MprB Extracytoplasmic Domain Negatively Regulates Activation of the Mycobacterium tuberculosis MprAB Two-Component System. Bretl, D, J. et al. J. Bacteriol. Jan 2014. 196: 391-406.
Zinc Finger Nuclease-Expressing Baculoviral Vectors Mediate Targeted Genome Integration of Reprogramming Factor Genes to Facilitate the Generation of Human Induced Pluripotent Stem Cells. Phang, R. Z. et al. Stem Cells Trans Med. Dec 2013. 2: 935-945.
Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells. Zhu, H. et al. Nucleic Acid Res. Oct 2013. 41: e180.
Blastomyces dermatidis septins CDC3, CDC10 and CDC12 impact the morphology of yeast and hyphae, but are not required for the phase transition. Marty, A. J. and Gauthier, G. M. Med Mycol. Jan 2013. 51: 93-102.
MprA and DosR Coregulate a Mycobacterium tuberculosis Virulence Operon Encoding Rv1813c and Rv1812c. Bretl, D. J. et al. Infect. Immun. Sept 2012. 80: 3018-3033.
Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors.
DNA AND CHROMOSOMES: Phosphorylation of ORC2 Protein Dissociates Origin Recognition Complex from Chromatin and Replication Origins. Lee, K. Y. et al. J. Biol. Chem. Apr 2012. 287: 11891-11898.
MOLECULAR BIOLOGY OF PATHOGENS: PepD Participates in the Myobacterial Stress Response Mediated through MprAB and SigE. Whtie, M. J. et al. J. Bacteriol. Mar 2010. 192: 1498-1510.
Fluorescent Tagging and Cellular Distribution of the Kaposi's Sarcoma-Associated Herpesvirus ORF45 Tegument Protein X Jia, S Shen, Y Lv, Z Zhang, H Guo, H Deng - Journal of Virology, 2016

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