Hygromycin B Solid | CAS 31282-04-9 | Antibiotics | AG Scientific, Inc

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Hygromycin B Solid, High Purity, Cell Culture Tested

  • CAS: 31282-04-9
  • Formula: C20H37N3O13
  • MW: 527.5 Da
  • Appearance: Cream colored powder
  • Purity: ≥90%




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Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for the selection of hygromycin resistance gene transformed cells. Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.

Antibiotics are often used in clinical in-vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. Hygromycin's longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.

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SynonymsHygrovetine, Hygrovectine, D-streptamine
Product #H-1012-SOLID
CAS #31282-04-9
Chemical NameO-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
MW527.5 Da
AppearanceCream colored powder
SolubilityWater (50 mg/mL): clear, light-tan solution
Preparation Working solutions (<2mg>
Storage TempStore at +4°C
Therapeutic AreaInfectious diseases
UseHygromycin B is an aminoglycoside antibiotic that kills bacteria and fungi.

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Merck Index13.4878
MDL NumberMFCD06795479

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GHS Pictograms
UN #'SUN 3462
HandlingWarning.Toxic! May be carcinogenic. May be teratogenic. Protect from moisture!

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msds 1H-1012, Hygromycin B, SDS, diamond format.pdf
Certificate of Analysis 1H-1012-SOLID, J1258.pdf
Certificate of Analysis 2H-1012-SOLID, J1047.pdf
Certificate of Analysis 3H-1012-SOLID, H1079A.pdf
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CitationsBriones-Martin-del-Campo, Marcela, et al. "The superoxide dismutases of Candida glabrata protect against oxidative damage and are required for lysine biosynthesis, DNA integrity and chronological life survival." Microbiology 161.2 (2015): 300-310.
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Armstrong, Richard M., et al. "Rv2744c is a PspA ortholog that regulates lipid droplet homeostasis and non-replicating persistence in Mycobacterium tuberculosis." Journal of bacteriology (2016): JB-01001.
Cokol, Murat, et al. "Systematic exploration of synergistic drug pairs." Molecular systems biology 7.1 (2011): 544.
Bretl, Daniel J., et al. "The MprB extracytoplasmic domain negatively regulates activation of the Mycobacterium tuberculosis MprAB two-component system." Journal of bacteriology (2013): JB-01064.
Misra, Ashish, and Michael R. Green. "Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators." Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation. Humana Press, New York, NY, 2017. 1-12.
Krumpe, Katrin, et al. "Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes." Molecular biology of the cell 23.20 (2012): 3927-3935.
Gallegos-Garci­a, Veronica, et al. "A novel downstream regulatory element cooperates with the silencing machinery to repress EPA1 expression in Candida glabrata." Genetics (2012): genetics-111.
Adeyo, Oludotun, et al. "The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets." The Journal of cell biology 192.6 (2011): 1043-1055.
Mahameed, Mohamed, and Boaz Tirosh. "Engineering CHO cells with an oncogenic KIT improves cells growth, resilience to stress, and productivity." Biotechnology and bioengineering 114.11 (2017): 2560-2570.
Kou, Yanjun, et al. "Structure-function analyses of the Pth11 receptor reveal an important role for CFEM motif and redox regulation in rice blast." New Phytologist 214.1 (2017): 330-342.
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Kim, Ahreum, et al. "Protective vaccine efficacy of the complete form of PPE39 protein from Mycobacterium tuberculosis Beijing/K strain in mice." Clinical and Vaccine Immunology (2017): CVI-00219.
Kleinovink, E. W. J. The use of light in cancer immunotherapy. Diss. 2018.
Kujoth, Gregory C., et al. "CRISPR/Cas9-Mediated Gene Disruption Reveals the Importance of Zinc Metabolism for Fitness of the Dimorphic Fungal Pathogen Blastomyces dermatitidis." mBio 9.2 (2018): e00412-18.
He, Hanjun, et al. "CELLULASE 6 and MANNANASE 7 affect cell differentiation and silique dehiscence." Plant physiology (2018): pp-01494.
Bergson, Shir, et al. "Fluorescent Tagging and Cellular Distribution of the Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Open Reading Frame 45 (ORF45) Tegument Protein." Journal of virology (2014): JVI-01091.
Samadder, Partha, et al. "Transcriptional and post-transcriptional enhancement of gene expression by the 5′ UTR intron of rice rubi3 gene in transgenic rice cells." Molecular Genetics and Genomics 279.4 (2008): 429-439.
ReferenceErgosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes
Human Telomerase Reverse Transcriptase (hTERT) Is a Novel Target of the Wnt/beta-Catenin Pathway in Human Cancer
A Novel Downstream Regulatory Element Cooperates with the Silencing Machinery to Repress EPA1 Expression in Candida glabrata
Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors. Edith S.A. Hofinger et al, Glycobiology. 2007. 17: 444-453.
Systematic exploration of synergised drug pairs. Cokol, M. et al. Mol Syst Biol. Jul 2014. 7: 544.
The MprB Extracytoplasmic Domain Negatively Regulates Activation of the Mycobacterium tuberculosis MprAB Two-Component System. Bretl, D, J. et al. J. Bacteriol. Jan 2014. 196: 391-406.
Zinc Finger Nuclease-Expressing Baculoviral Vectors Mediate Targeted Genome Integration of Reprogramming Factor Genes to Facilitate the Generation of Human Induced Pluripotent Stem Cells. Phang, R. Z. et al. Stem Cells Trans Med. Dec 2013. 2: 935-945.
Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells. Zhu, H. et al. Nucleic Acid Res. Oct 2013. 41: e180.
Blastomyces dermatidis septins CDC3, CDC10 and CDC12 impact the morphology of yeast and hyphae, but are not required for the phase transition. Marty, A. J. and Gauthier, G. M. Med Mycol. Jan 2013. 51: 93-102.
MprA and DosR Coregulate a Mycobacterium tuberculosis Virulence Operon Encoding Rv1813c and Rv1812c. Bretl, D. J. et al. Infect. Immun. Sept 2012. 80: 3018-3033.
Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors.
DNA AND CHROMOSOMES: Phosphorylation of ORC2 Protein Dissociates Origin Recognition Complex from Chromatin and Replication Origins. Lee, K. Y. et al. J. Biol. Chem. Apr 2012. 287: 11891-11898.
MOLECULAR BIOLOGY OF PATHOGENS: PepD Participates in the Myobacterial Stress Response Mediated through MprAB and SigE. Whtie, M. J. et al. J. Bacteriol. Mar 2010. 192: 1498-1510.
Fluorescent Tagging and Cellular Distribution of the Kaposi's Sarcoma-Associated Herpesvirus ORF45 Tegument Protein X Jia, S Shen, Y Lv, Z Zhang, H Guo, H Deng - Journal of Virology, 2016

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