IPTG | CAS 367-93-1 | Inducer of beta-galactosidase activity | AG Scientific, Inc.

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IPTG

I-1312
  • CAS: 367-93-1
  • Formula: C9H18O5S
  • MW: 238.3 Da
  • Appearance: White crystalline powder
  • Purity: >99%

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IPTG (i-Propyl-β-D-ThioGalactoPyranoside) is utilized for the induction of expression from the lac promoter and derivates. IPTG is an inducer of galactosidase activity in many bacteria. Functioning as an analog, IPTG induces galactosidase activity by binding to and inhibiting the repressor. IPTG is used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene. Dissolve IPTG in water at 200 mg/ml. Sterilize solution through a 0.2 μm filter, dispense in small aliquots and store at -20°C. Use at a final concentration of 250-350 μg/mL in top agar and liquid cultures. Specific applications may require more or less IPTG. Isopropyl-β-D-thiogalactoside is analytical grade material, free of dioxane.

Additional Information

SynonymsIsopropyl-β-D-thiogalactopyranoside, Isopropyl β-D-1-thiogalactopyranoside; Isopropyl-β-D-thiogalactoside
Product #I-1312
CAS #367-93-1
Chemical Nameisopropyl-β-d-thiogalactopyranoside
FormulaC9H18O5S
MW238.3 Da
AppearanceWhite crystalline powder
Purity>99%
SolubilitySoluble in water (5%), renders clear colorless solution
Melting Point111 - 113ºC
Storage TempStore at -20°C
UseAn analogue of allolactose that binds specifically to the repressor protein of the lac operon.

Additional Information

MDL NumberMFCD00063273
ChemACXX1007022-5
InChIInChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
SMILESCC(C)S[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O

Additional Information

GHS Pictograms
HandlingStore in a tightly sealed container. Protect from light and moisture. Smoking, eating and drinking should be prohibited in the application area.

Additional Information

msds 1I-1312, IPTG, SDS, diamond format.pdf
Certificate of Analysis 1I-1312, J1109.pdf
Certificate of Analysis 2I-1312, J1080A.pdf
Certificate of Analysis 3I-1312, J1080.pdf
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