Proteinase K | Serine Protease | CAS 39450-01-6 | AG Scientific, Inc.

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Proteinase K

P-1265
  • CAS: 39450-01-6
  • MW: 28,500 Da
  • Appearance: White to off-white lyophilized solid

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Description

Proteinase K (also known as endopeptidase K) is an extracellular endopeptidase isolated from a culture filtrate of the fungus Tritirachium album limber. This unique fungus can degrade keratin (hence Proteinase “K”) and can grow using the by-product as its sole source of carbon and nitrogen.

The enzyme is stable over a broad temperature and pH range, with optimum activity at 55-65°C and pH 8.0. It’s also stable in high salt concentrations and active in the presence of strong detergents such as SDS. It has a molecular weight of 28.5 kDa and it shows fairly-low specificity for its polypeptide targets.

 

Features and Benefits of AG’s Proteinase K

• DNAse and RNAse free via chromatography purification
• 6x greater activity than Pronase and 3x greater activity than bovine trypsin
• Broad substrate specificity
• Versatile in blood, cultured cells, tissues, bacteria, yeast, etc.
• Active and stable over a wide range of conditions
• Compatible with most DNA and RNA isolation buffers

 

Applications in Next Generation Sequencing (NGS) and Microarray Technologies

• Nucleic acid purification by inactivating nucleases when extracting DNA and RNA from yeast, bacteria, and mammalian cell & plant cell lysates
• Improves cloning efficiency of PCR products
• Sample preparation for quantification of DNA adduct levels by accelerator mass spectrometry
• Inactivation of enzyme cocktails in ribonuclease protection assays
• Added to extraction procedures to optimize RNA yields from primary breast tumors for microarray studies

 

Applications with Proteins

• Detection of Bovine Spongiform Encephalopathy proteins which are uniquely resistant to proteolytic degradation.
• Tissue digestion as an alternative sample preparation approach for quantitative analysis using liquid chromatography—tandem mass spectrometry
• Specifically modify cell surface proteins to analyze membrane structures for protein localization
• Generates protein fragments used in characterization of functional studies

 

Specifications

• EC Number: 3.4.21.64 

 

Additional Information

SynonymsEndopeptidase K, Protease K
Product #P-1265
CAS #39450-01-6
MW28,500 Da
AppearanceWhite to off-white lyophilized solid
Solubility50mM Tris-HCl, 2mM calcium acetate, pH 8.0
Storage TempStore desiccated at +4°C. Store in tightly sealed vial.
Therapeutic AreaMolecular biology
UseProteinase K is a stable and highly reactive serine protease useful for the inactivation of nucleases in DNA and RNA isolation.

Additional Information

MDL NumberMFCD00132129
ChemACXX1010077-5

Additional Information

GHS Pictograms
HandlingStore desiccated at +4°C. Store in tightly sealed vial. WARNING: HARMFUL! IRRITANT!

Additional Information

msds 1P-1265, Proteinase K, SDS, diamond format.pdf
Certificate of Analysis 1P-1265, J1298.pdf
Certificate of Analysis 2P-1265, J1072I.pdf
Certificate of Analysis 3P-1265, J1072H.pdf
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Additional Information

CitationsZarban, Ali, et al. "Localization and Distribution of Primary Cilia In the Adult Mouse Heart." Heart and Cardiology, vol. 2, no. 2, 7 Nov. 2016.
ReferenceHoffmann, Marcus, et al. "Site-Specific O-Glycosylation Analysis of Human Blood Plasma Proteins." Molecular & Cellular Proteomics, vol. 15, no. 2, 23 Nov. 2015, pp. 624-641., doi:10.1074/mcp.m115.053546.
Silva, Lucas P., et al. "Evaluation of Two Novel Leptospiral Proteins for Their Interaction with Human Host Components." Pathogens and Disease, vol. 74, no. 5, 28 Apr. 2016, doi:10.1093/femspd/ftw040.
Llorens, Franc, et al. "Identification of New Molecular Alterations in Fatal Familial Insomnia." Human Molecular Genetics, vol. 25, no. 12, 7 Apr. 2016, pp. 2417-2436., doi:10.1093/hmg/ddw108.
Rockenstein, Edward, et al. "Glucocerebrosidase Modulates Cognitive and Motor Activities in Murine Models of Parkinson's Disease." Human Molecular Genetics, vol. 25, no. 13, 28 Apr. 2016, pp. 2645-2660., doi:10.1093/hmg/ddw124.
Suzuki, Mari, et al. "Glucocerebrosidase Deficiency Accelerates the Accumulation of Proteinase K-Resistant α-Synuclein and Aggravates Neurodegeneration in a Drosophila model of Parkinson's Disease." Human Molecular Genetics, vol. 24, no. 23, 11 Sept. 2015, pp. 6675-6686., doi:10.1093/hmg/ddv372.
Localization and distribution of primary cilia in adult mouse heart

Additional Information

Additional Videos
What is proteinase K used for?Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid.
What is the quickest most effective way to inactivate proteinase K?As with most protein enzymes, change the temperature or change the pH significantly.

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