Proteinase K

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Proteinase K P-1265
  • CAS: 39450-01-6
  • MW: 28,500 Da
  • Appearance: White to off-white lyophilized solid

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Proteinase K is a nonspecific serine protease used in the following applications: inactivation of Rnase and Dnases, isolation of high molecular weight DNA, isolation of RNA or the isolation of plasmid and genomic DNA. Proteinase K is used for the isolation of native high molecular genomic nucleic acids. Enzymes like DNases and RNases from microorganisms and mammalian cells are rapidly inactivated by Proteinase K.

Adding Proteinase K already during the cell lysis enables the isolation of highly native undamaged high molecular DNA or RNA. A variety of methods have been established, which are documented in numerous publications. Recently, Proteinase K has been used for the detection of BSE forming proteins which are uniquely resistant towards the enzyme's proteolytic cleavage.

Proteinase K is very useful in the analysis of membrane structure by means of modification of proteins and glycoproteins on cell surfaces. Because of the cleavage specificity Proteinase K, characteristic fragments of proteins are obtained which are helpful in revealing the structure and function of proteins, particularly enzymes.

 

Additional Information

Product #P-1265
CAS #39450-01-6
MW28,500 Da
AppearanceWhite to off-white lyophilized solid
Solubility50mM Tris-HCl, 2mM calcium acetate, pH 8.0
Storage TempStore desiccated at +4°C. Store in tightly sealed vial.
UseProteinase K is a stable and highly reactive serine protease

Additional Information

MDL NumberMFCD00132129

Additional Information

GHS Pictograms
HandlingStore desiccated at +4°C. Store in tightly sealed vial.
Hazard ImageYes

Additional Information

msds 1P-1265, SDS.pdf
Certificate of Analysis 1P-1265, H1069H.pdf
Certificate of Analysis 2P-1265, H1335.pdf
Certificate of Analysis 3P-1265, H1281.pdf

Additional Information

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