Puromycin, dihydrochloride


More Views

Puromycin, dihydrochloride P-1033
Promotion ends on Nov 30th. Use Code: 25ANTI
  • CAS: 58-58-2
  • Formula: C22H29N7O5 2HCl
  • MW: 544.4
  • Purity: >98% by HPLC





Add to Cart Bulk Inquiry

Puromycin Dihydrochloride is the hydrochloride salt form of puromycin, an aminoglycoside antibiotic isolated from the bacterium Streptomyces alboniger. Acting as an analog of the 3' terminal end of aminoacyl-tRNA, puromycin incorporates itself into a growing polypeptide chain and causes its premature termination, thereby inhibiting protein synthesis. This agent has antimicrobial, antitrypanosomal, and antineoplastic properties. Puromycin's main application is in cell biology as a selective antibiotic agent in cell culture systems for selecting mammalian cell lines, which have been transformed by vectors that express puromycin-N-acetyl-transferase (PAC). Puromycin’s longstanding application as a gene selection antibiotic has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

Recently researchers have developed a prodrug strategy for selective cancer therapy using a masked cytotoxic agent Fmoc-Lys(Ac)-Puromycin, which can be sequentially activated by histone deacetylases (HDACs) and cathepsin L (CTSL) to kill cancer cells expressing high levels of both enzymes. The data gathered indicates that Fmoc-Lys(Ac)-Puromycin can substantially improve anti cancer efficacy and can be developed into potential selective cancer therapy.


Additional Information

SynonymsStylomycin dihydrochloride, CL 13900 dihydrochloride, CL16536, NSC 3055, P638 dihydrochloride.
Product #P-1033
CAS #58-58-2
Chemical Name(S) 3'-[[2-Amino-3-(4-methoxyphenyl)-1-oxopropyl]amino]-3'-deoxy-N,N-dimethyladenosine,3'-(a-Amino-p-methoxy-hydocinnamamido)-3'-dexoy-N,N-dimethyladenosine.2HCl
FormulaC22H29N7O5 2HCl
Purity>98% by HPLC
SolubilitySoluble in water (50 mg/ml), ethanol (~1 mg/ml), DMSO (~13 mg/ml), DMF (~14 mg/ml), and PBS, pH 72 (~10 mg/ml)
Melting Point175-177 °C
Boiling Point752.16 °C
Storage Temp-20°C
Therapeutic AreaInfectious Diseases
UseAn aminonuclease antibiotic used for selection and maintenance of cell lines expressing a transfected pac gene

Additional Information

Merck Index14: 7943
MDL NumberMFCD00866328

Additional Information

UN #'SUN 2811

Additional Information

msds 1P-1033, SDS.pdf
Certificate of Analysis 1P-1033, H1215C.pdf
Certificate of Analysis 2P-1033, H1215B.pdf
Certificate of Analysis 3P-1033, H1215A.pdf

Additional Information

CitationsCELL BIOLOGY:\n CBP and p300 are cytoplasmic E4 polyubiquitin ligases for p53\n Dingding Shi, Marius S. Pop, Roman Kulikov, Ian M. Love, Andrew L. Kung, and Steven R. Grossman\n PNAS, Sep 2009; 106: 16275 - 16280.
NEOPLASIA:\n High TCL1 expression and intact T-cell receptor signaling define a hyperproliferative subset of T-cell prolymphocytic leukemia\n Marco Herling, Kaushali A. Patel, Michael A. Teitell, Marina Konopleva, Farhad Ravandi, Ryuji Kobayashi, and Dan Jones\n Blood, Jan 2008; 111: 328 - 337.
Ligand-Independent Regulation of Transforming Growth Factor 1 Expression and Cell Cycle Progression by the Aryl Hydrocarbon Receptor - Xiaoqing Chang, Yunxia Fan, Saikumar Karyala, Sandy Schwemberger, Craig R. Tomlinson, Maureen A. Sartor, and Alvaro Puga
The mTORC1 signaling repressors REDD1/2 are rapidly induced and activation of p70S6K1 by leucine is defective in skeletal muscle of an immobilized rat hindlimb\n Andrew R. Kelleher, Scot R. Kimball, Michael D. Dennis, Rudolf J. Schilder, and Leonard S. Jefferson\n Am J Physiol Endocrinol Metab, Jan 2013; 304: E229 - E236.
Inhibition of Gastric Cancer Invasion and Metastasis by PLA2G2A, a Novel Beta-Catenin/TCF Target Gene Kumaresan Ganesan, Tatiana Ivanova, Yonghui Wu, Vikneswari Rajasegaran, Jeanie Wu, Ming Hui Lee, Kun Yu, Sun Young Rha, Hyun Cheol Chung, Bauke Ylstra, Gerrit Meijer, Kon Oi Lian, Heike Grabsch, and Patrick Tan
The Aryl Hydrocarbon Receptor Binds to E2F1 and Inhibits E2F1-induced Apoptosis\n Jennifer L. Marlowe, Yunxia Fan, Xiaoqing Chang, Li Peng, Erik S. Knudsen, Ying Xia, and Alvaro Puga\n Mol. Biol. Cell, Aug 2008; 19: 3263 - 3271.
STRUCTURE AND ASSEMBLY:\n Fluorescent Tagging and Cellular Distribution of the Kaposi's Sarcoma-Associated Herpesvirus ORF45 Tegument Protein\n Shir Bergson, Inna Kalt, Inbal Itzhak, Kevin F. Brulois, Jae U. Jung, and Ronit Sarid\n J. Virol., Nov 2014; 88: 12839 - 12852.
PATHOGENESIS AND IMMUNITY:\n Hepatitis C Virus Entry Is Impaired by Claudin-1 Downregulation in Diacylglycerol Acyltransferase-1-Deficient Cells\n Pil Soo Sung, Asako Murayama, Wonseok Kang, Myung-Sun Kim, Seung Kew Yoon, Masayoshi Fukasawa, Masuo Kondoh, Jin-Soo Kim, Hyongbum Kim, Takanobu Kato, and Eui-Cheol Shin\n J. Virol., Aug 2014; 88: 9233 - 9244.
Exosome-derived miRNAs and ovarian carcinoma progression - Olga Vaksman Claes Tropé Ben Davidson Reuven Reich
ReferenceThe Aryl Hydrocarbon Receptor Binds to E2F1 and Inhibits E2F1-induced Apoptosis
High TCL1 expression and intact T-cell receptor signaling define a hyperproliferative subset of T-cell prolymphocytic leukemia
AG490 and PF431396 Sensitive Tyrosine Kinase Control the Population Heterogeneity of Basal STAT1 Activity in Ube1l Deficient Cells - Hesung Now, Joo-Yeon Yoo

Additional Information


Got Questions? We have Answers!

Our team of experts are standing by to help you in every possible way.

Real People, Real Time, Rapid Response

Chip Lindgren


Ken Smith


Kyle Anderson

Procurement & Business Development

Amrutha Karuturi

Web Content and Marketing

Lee DeCicco

Customer Service


* Required Fields

*Bulk OEM Multiple Catalog Custom Other
Prod#CAS#*PRODUCT NAMEGrade*Size*QtyTime Frame

Custom magento captcha module from Outsource Online