Puromycin | CAS 58-58-2 | Gene Selection Antibiotic | AG Scientific, Inc.

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Puromycin Dihydrochloride Solution

P-1033-SOL
  • CAS: 58-58-2
  • Formula: C22H29N7O5 • 2HCl
  • MW: 544.43 Da
  • Appearance: Clear and colorless to light yellow solution
  • Purity: ≥99%

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DESCRIPTION

AG Scientific's Puromycin dihydrochloride solution is a sterile filtrate solution.

Puromycin is an aminonucleoside antibiotic that causes early chain termination within the ribosome during translation. This inhibition of translation is effective in both in vivo and in vitro applications, making it an ideal tool for protein synthesis studies. While selection for E. Coli cells is possible with puromycin, it is more commonly and effectively used for mammalian cell selection.

It is active against gram-positive microorganisms, less active against acid-fast bacilli and more-weakly active against gram-negative microorganisms. The growth of bacteria, protozoa, algae and mammalian cells is quickly halted by puromycin. However, cells with the pac gene have resistance, its gene product is puromycin-N-acetyl-transferase which inactivates puromycin through acetylation. Research has shown puromycin to be effective against certain tumors.

Recently researchers have developed a prodrug strategy for selective cancer therapy using a masked cytotoxic agent Fmoc-Lys(Ac)-Puromycin, which can be sequentially activated by histone deacetylases (HDACs) and cathepsin L (CTSL) to kill cancer cells expressing high levels of both enzymes. The data gathered indicates that Fmoc-Lys(Ac)-Puromycin can substantially improve anti-cancer efficacy and can be developed into potential selective cancer therapy.

 

APPLICATIONS

• Puromycin is soluble in water and is often used as a selective agent in molecular biology research.
• Puromycin has a longstanding application as a gene selection antibiotic and a powerful new role in Genome editing utilizing the CRISPR/Cas9 system.

 

SPECIFICATIONS

Purity data is for a concentration of 10 mg/ml.

 

Additional Information

SynonymsStylomycin, P638, Puromycin solution
Product #P-1033-SOL
CAS #58-58-2
Chemical Name(S) 3'-[[2-Amino-3-(4-methoxyphenyl)-1-oxopropyl]amino]-3'-deoxy-N,N-dimethyladenosine,3'-(a-Amino-p-methoxy-hydocinnamamido)-3'-dexoy-N,N-dimethyladenosine.2HCl
FormulaC22H29N7O5 • 2HCl
MW544.43 Da
AppearanceClear and colorless to light yellow solution
Purity≥99%
SolubilitySoluble in DMSO (1M).
Storage Temp-20°C
Therapeutic AreaInfectious diseases
UsePuromycin dihydrochloride is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium

Additional Information

Merck Index13.8044.2001
MDL NumberMFCD00012691
ChemACXX1017033-1
InChIInChI=1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1
SMILESCN(C)c1c2c(ncn1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](Cc4ccc(cc4)OC)N)O.Cl.Cl

Additional Information

GHS Pictograms
HandlingWarning.Toxic. May be Carcinogenic. Wear gloves/mask when handling product. Avoid contact by all means of exposure. Protect from Moisture.

Additional Information

msds 1P-1033, Puromycin dihydrochloride, SDS, diamond format.pdf
Certificate of Analysis 1P-1033-SOL, H1401A.pdf
Certificate of Analysis 2P-1033-SOL, H1401.pdf
Certificate of Analysis 3P-1033-SOL, H1384.pdf
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Additional Information

CitationsSeo-hee, Nam. Novel Functions of Lysyl-TRNA Synthetase in Invasive Dissemination of Colon Cancer Cells in 3D Microenvironment. Edited by Lee Jung Won, Seoul National University Graduate School, 2017.
Bergson, Shir, et al. "Fluorescent Tagging and Cellular Distribution of the Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Open Reading Frame 45 (ORF45) Tegument Protein." Journal of virology (2014): JVI-01091.
Now, Hesung, and Joo-Yeon Yoo. "AG490 and PF431396 sensitive tyrosine kinase control the population heterogeneity of basal STAT1 activity in Ube1l deficient cells." PloS one 11.7 (2016): e0159453.
Gelgor, Anastasia, et al. "Nucleolar stress enhances lytic reactivation of the Kaposi's sarcoma-associated herpesvirus." Oncotarget 9.17 (2018): 13822.
Zurawski, Gerard, et al. "Antigen presenting cell targeted cancer vaccines." U.S. Patent Application No. 14/819,909.
Brody, Yehuda, et al. "The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing." PLoS biology 9.1 (2011): e1000573.
Ganesan, Kumaresan, et al. "Inhibition of gastric cancer invasion and metastasis by PLA2G2A, a novel b-catenin/TCF target gene." Cancer research 68.11 (2008): 4277-4286.
Weitzenfeld, Polina, et al. "Chemokine axes in breast cancer: factors of the tumor microenvironment reshape the CCR7-driven metastatic spread of luminal-A breast tumors." Journal of leukocyte biology 99.6 (2016): 1009-1025.
Herling, Marco, et al. "High TCL1 expression and intact T-cell receptor signaling define a hyperproliferative subset of T-cell prolymphocytic leukemia." Blood 111.1 (2008): 328-337.
ReferenceThe Aryl Hydrocarbon Receptor Binds to E2F1 and Inhibits E2F1-induced Apoptosis, Jennifer L. Marlowe, Yunxia Fan, et al, Aug 2008; 19: 3263 - 3271.
High TCL1 expression and intact T-cell receptor signaling define a hyperproliferative subset of T-cell prolymphocytic leukemia, Marco Herling, Kaushali A. Patel, et al, Sep 2008;111:328-337
CBP and p300 are cyto plasmic E4 polyubiquitin ligases for p53, Dingding Shi, Marius S. Pop, et al, Sep 2009;vol. 106 no. 38:16275-16280
CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani
A Comprehensive Toolbox for Genome Editing in Cultured Drosophila Cells

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