5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.
X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity.
X-gal is used to indicate whether a cell expresses the β-glactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue white screening. Bacterial cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.
|Synonyms||BCIG, 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside|
|Solubility||Soluble 2% in DMF|
|Melting Point||236-238 °C|
|Use||A chromogenic histochemical substrate for β-galactosidase used to detect DNA insertions into the lacZ region of plasmid DNA|
|msds 1||X-1015, X-Gal, SDS, diamond format.pdf|
|Certificate of Analysis 1||X-1015, H1100A.pdf|
|Certificate of Analysis 2||X-1015, H1100.pdf|
|Certificate of Analysis 3||X-1015, G1140.pdf|
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