X-GAL

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X-GAL

X-1015
  • CAS: 7240-90-6
  • Formula: C14H15BrClNO6
  • MW: 408.61
  • Appearance: White Powder
  • Purity: 99% (TLC)

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5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.

X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity.

X-gal is used to indicate whether a cell expresses the β-glactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue white screening. Bacterial cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.

 

 

 

Additional Information

SynonymsBCIG
Product #X-1015
CAS #7240-90-6
Chemical Name5-Bromo-4-Chloro-3-Indolyl-B-D-Galactopyranoside
FormulaC14H15BrClNO6
MW408.61
AppearanceWhite Powder
Purity99% (TLC)
SolubilitySoluble 2% in DMF
Melting Point236-238 °C
Storage Temp-20°C
UseA chromogenic histochemical substrate for β-galactosidase used to detect DNA insertions into the lacZ region of plasmid DNA

Additional Information

MDL NumberMFCD00005666
ChemACXX1009969-1
InChIInChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
SMILESc1cc(c(c2c1[nH]cc2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br

Additional Information

Additional Information

msds 1X-1015, X-Gal, SDS, diamond format.pdf
Certificate of Analysis 1X-1015, H1100A.pdf
Certificate of Analysis 2X-1015, H1100.pdf
Certificate of Analysis 3X-1015, G1140.pdf
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